New sensitive bromatometric assay methods for the determination of four analgesic drugs in pharmaceutical formulations and biological fluids

New sensitive and rapid spectrophotometric methods for the determination of four analgesic drugs namely, nalbuphine (NALB), naltrexone (NALT), morphine (MORF) and tramadol (TRAM) in pharmaceutical formulations and biological fluids were developed and optimized. The proposed methods involve the addit...

Full description

Saved in:
Bibliographic Details
Published in:Journal of pharmaceutical education and research 2013-06, Vol.4 (1), p.54
Main Authors: El-didamony, Akram M, Khater, Hanaa M, Ali, Ismail I
Format: Article
Language:English
Subjects:
Analytical chemistry
Chromatography
Methods
Narcotics
Pain
Pharmaceuticals
Scientific Concepts
Water
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:New sensitive and rapid spectrophotometric methods for the determination of four analgesic drugs namely, nalbuphine (NALB), naltrexone (NALT), morphine (MORF) and tramadol (TRAM) in pharmaceutical formulations and biological fluids were developed and optimized. The proposed methods involve the addition of a measured excess of bromate-bromide mixture in acid medium and subsequent estimation of the residual bromine by reacting with either a fixed amount of indigo carmine and measuring the absorbance at 610 nm, or amaranth and measuring the absorbance at 520 nm. In both methods, the amount of bromine reacted corresponds to the amount of drugs. Under the optimum conditions, Beer's law limit, molar absorptivity and Sandell's sensitivity were calculated. The limits of detection and quantification were also reported for both methods. Statistical evaluation of the methods was examined by determining an intra-day and inter-day precisions. The methods were successfully applied to the assay of drugs in their pharmaceutical formulations and biological fluids. No interference was observed from common additives and the validity of the methods was tested. [PUBLICATION ABSTRACT]
ISSN:0976-8173
0976-8238