DEVELOPMENT AND VALIDATION OF A TLC METHOD FOR THE ANALYSIS OF SYNTHETIC FOOD-STUFF DYES

A novel reversed-phase thin-layer chromatographic (RP-TLC) method was developed for simultaneous determination of ten most commonly used artificial food dyes. The method was validated with respect to specificity, linearity, precision, and accuracy and compared with reversed-phase high performance li...

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Bibliographic Details
Published in:Journal of liquid chromatography & related technologies 2013-10, Vol.36 (17), p.2476-2488
Main Authors: Vlajkovic, Jelena, Andric, Filip, Ristivojevic, Petar, Radoicic, Aleksandra, Tesic, Zivoslav, Milojkovic-Opsenica, Dusanka
Format: Article
Language:English
Subjects:
Ammonium acetate
Chromatography
Dyes
Food
Food quality control
Foods
green analytical chemistry
Linearity
Liquid chromatography
Mass spectrometry
method development
reversed-phase high-performance liquid chromatography
reversed-phase thin-layer chromatography
Silicon dioxide
synthetic food colorants
Wavelengths
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Summary:A novel reversed-phase thin-layer chromatographic (RP-TLC) method was developed for simultaneous determination of ten most commonly used artificial food dyes. The method was validated with respect to specificity, linearity, precision, and accuracy and compared with reversed-phase high performance liquid chromatography (RP-HPLC) method. In the case of TLC method horizontal developing technique was employed using octadecyl-modified silica as a stationary phase and 0.5 mol dm −3 ammonium sulfate in 30% of ethanol-water solution as a mobile phase. HPLC analysis was carried out using XDB-C18 column and gradient of mixture of aqueous ammonium acetate solution (0.1 mol dm −3 , pH = 6.5) and MeOH/ACN (1:1, v/v). Colorants were detected at 450, 500, and 625 nm wavelengths. The TLC method proved to be suitable for quantitative determination in relatively wide range of concentrations (20-300 ng/band). Satisfactory values of limits of detection and quantitation were established for both techniques, although somewhat higher in the case of TLC, ranging from 2-10 ng/band, that is, 3-20 ng/band. Both methods can be applied with satisfactory precision and accuracy, with clear advantage of TLC for simultaneous analysis of six samples per hour.
ISSN:1082-6076
1520-572X
DOI:10.1080/10826076.2013.790771