Thiol Antioxidants in Combination with Vitamin B^sub 12^ Induce Apoptotic Death of Human Lymphocytic Leukemia Cells by Destabilization of Lysosomes with the Involvement of Iron Ions

Issue Title: This issue is a translation of Byulleten' Eksperimental'noi Biologii i Meditsiny (Bulletin of Experimental Biology and Medicine) and Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine) The extensively used thiol antioxidants (dithiothreito...

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Published in:Bulletin of experimental biology and medicine 2013-02, Vol.154 (4), p.449
Main Authors: Solovyeva, M E, Faskhutdinova, A A, Solovyev, V V, Akatov, V S
Format: Article
Language:English
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Summary:Issue Title: This issue is a translation of Byulleten' Eksperimental'noi Biologii i Meditsiny (Bulletin of Experimental Biology and Medicine) and Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine) The extensively used thiol antioxidants (dithiothreitol, glutathione, and N-acetylcysteine) in combination with hydroxycobalamine (vitamin B^sub 12^) gain toxic activity in relation to human lymphocytic leukemia cell line HL60. Combined treatment with thiol and vitamin B12 was followed by early destabilization of lysosomes and apoptotic death of cells. The cytotoxic effect was abolished by caspase inhibitors. An iron-chelating agent deferoxamine partly prevented cell death, while lysosomal protease inhibitor pepstatin produced no protective effect.[PUBLICATION ABSTRACT]
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-013-1974-y