Mifepristone Treatment Results in Differential Regulation of Glycerolipid Biosynthesis in Baby Hamster Kidney Cells Expressing a Mifepristone-Inducible ABCA1

ATP binding cassette A1 (ABCA1) transports cholesterol, phospholipids and lipophilic molecules to and across cellular membranes. We examined if ABCA1 expression altered cellular de novo glycerolipid biosynthesis in growing Baby hamster kidney (BHK) cells. Mock BHK cells or cells expressing a mifepri...

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Bibliographic Details
Published in:Lipids 2011-09, Vol.46 (9), p.795-804
Main Authors: Hauff, Kristin D., Mitchell, Ryan W., Xu, Fred Y., Dembinski, Thomas, Mymin, David, Zha, Xiaohui, Choy, Patrick C., Hatch, Grant M.
Format: Article
Language:English
Subjects:
ABCA1
Amino acid
Animals
ATP Binding Cassette Transporter 1
ATP binding cassette‐1
ATP-Binding Cassette Transporters - genetics
ATP-Binding Cassette Transporters - metabolism
BHK cells
Biomedical and Life Sciences
Biosynthesis
Cell Line
Cholesterol transport
Cricetinae
Diacylglycerol acyltransferase
Diacylglycerol O-Acyltransferase - genetics
Diacylglycerol O-Acyltransferase - metabolism
Enzyme Assays
Gene Expression - drug effects
Glycerides - biosynthesis
Glycerol - metabolism
Glycerophospholipids - metabolism
Histone Deacetylase Inhibitors - pharmacology
Hydroxamic Acids - pharmacology
Inositol
Inositol - metabolism
Kidneys
Life Sciences
Lipidology
Medical Biochemistry
Medicinal Chemistry
Microbial Genetics and Genomics
Mifepristone
Mifepristone - pharmacology
Neurochemistry
Nutrition
Oleic Acid - metabolism
Original Article
Phosphatidylserines - biosynthesis
Phospholipid synthesis
Radioactivity
Serine
Serine - metabolism
Triacylglycerol
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Summary:ATP binding cassette A1 (ABCA1) transports cholesterol, phospholipids and lipophilic molecules to and across cellular membranes. We examined if ABCA1 expression altered cellular de novo glycerolipid biosynthesis in growing Baby hamster kidney (BHK) cells. Mock BHK cells or cells expressing a mifepristone-inducible ABCA1 (ABCA1) were incubated plus or minus mifepristone and then with [ 3 H]serine or [ 3 H]inositol or [ 3 H]ethanolamine or [ methyl - 3 H]choline or [ 3 H]glycerol or [ 14 C]oleate and radioactivity incorporated into glycerolipids determined. Mifepristone did not affect [1,3- 3 H]glycerol or [ 14 C]oleate or [ 3 H]ethanolamine or [ methyl - 3 H]choline uptake in BHK cells. In contrast, [ 3 H]glycerol and [ 14 C]oleate incorporated into phosphatidylserine (PtdSer) were elevated 2.4-fold ( p  
ISSN:0024-4201
1558-9307
DOI:10.1007/s11745-011-3582-9