Biodegradable Nanocapsules as siRNA Carriers for Mutant K-Ras Gene Silencing of Human Pancreatic Carcinoma Cells

The application of small interfering RNA (siRNA)‐based RNA interference (RNAi) for cancer gene therapy has attracted great attention. Gene therapy is a promising strategy for cancer treatment because it is relatively non‐invasive and has a higher therapeutic specificity than chemotherapy. However, w...

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Published in:Small (Weinheim an der Bergstrasse, Germany) Germany), 2013-08, Vol.9 (16), p.2757-2763
Main Authors: Lin, Guimiao, Hu, Rui, Law, Wing-Cheung, Chen, Chih-Kuang, Wang, Yucheng, Li Chin, Hui, Nguyen, Quoc Toan, Lai, Cheng Kee, Yoon, Ho Sup, Wang, Xiaomei, Xu, Gaixia, Ye, Ling, Cheng, Chong, Yong, Ken-Tye
Format: Article
Language:English
Subjects:
biodegradable
Cancer
Cancer therapies
Cell Line, Tumor
Gene Silencing - physiology
Gene therapy
Genes, ras - genetics
Humans
Nanocapsules - chemistry
Nanotechnology
pancreatic cancer
Pancreatic Neoplasms
Pancreatic Neoplasms - therapy
polymer nanocapsules
RNA, Small Interfering - genetics
RNA, Small Interfering - physiology
siRNA
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Summary:The application of small interfering RNA (siRNA)‐based RNA interference (RNAi) for cancer gene therapy has attracted great attention. Gene therapy is a promising strategy for cancer treatment because it is relatively non‐invasive and has a higher therapeutic specificity than chemotherapy. However, without the use of safe and efficient carriers, siRNAs cannot effectively penetrate the cell membranes and RNAi is impeded. In this work, cationic poly(lactic acid) (CPLA)‐based degradable nanocapsules (NCs) are utilized as novel carriers of siRNA for effective gene silencing of pancreatic cancer cells. These CPLA‐NCs can readily form nanoplexes with K‐Ras siRNA and over 90% transfection efficiency is achieved using the nanoplexes. Cell viability studies show that the nanoparticles are highly biocompatible and non‐toxic, indicating that CPLA‐NC is a promising potential candidate for gene therapy in a clinical setting. Cationic poly(lactic acid) (CPLA)‐based degradable nanocapsules (NCs) are utilized as novel carriers of siRNA for effective gene silencing of pancreatic cancer cells. These CPLA‐NCs can readily form nanoplexes with K‐Ras siRNA and over 90% transfection efficiency is achieved using the nanoplexes.
ISSN:1613-6810
1613-6829
DOI:10.1002/smll.201201716