Attenuation of adjuvant-induced arthritis by dietary sesamol via modulation of inflammatory mediators, extracellular matrix degrading enzymes and antioxidant status

• Published in: European journal of nutrition 2013-10, Vol.52 (7), p.1787-1799
• Main Authors: Hemshekhar, Mahadevappa, Thushara, Ram Mohan, Jnaneshwari, Sadashivaiah, Devaraja, Sannaningaiah, Kemparaju, Kempaiah, Girish, Kesturu Subbaiah
• Format: Article
• Language: English
• Subjects: acid phosphatase; Alanine Transaminase - blood; Animals; Anti-Inflammatory Agents - administration & dosage; antioxidants; Antioxidants - administration & dosage; arthritis; Arthritis, Experimental - drug therapy; Aspartate Aminotransferases - blood; Benzodioxoles - administration & dosage; blood serum; bone resorption; cardiovascular diseases; cartilage; catalase; Catalase - metabolism; cathepsin D; Chemistry; Chemistry and Materials Science; Cyclooxygenase 2 - metabolism; diabetes; extracellular matrix; Extracellular Matrix - metabolism; gelatinase A; glutathione transferase; Glutathione Transferase - metabolism; homeostasis; hyaluronoglucosaminidase; hydrogen peroxide; Hydrogen Peroxide - metabolism; inflammation; Inflammation Mediators - metabolism; interleukin-1beta; Interleukin-1beta - metabolism; interleukin-6; Interleukin-6 - metabolism; Matrix Metalloproteinase 13 - genetics; Matrix Metalloproteinase 13 - metabolism; Matrix Metalloproteinase 9 - genetics; Matrix Metalloproteinase 9 - metabolism; neutralization; Nutrition; Original Contribution; oxidative stress; Oxidative Stress - drug effects; Phenols - administration & dosage; Rats; Rats, Wistar; Reactive Oxygen Species - metabolism; sesame seed; superoxide dismutase; Superoxide Dismutase - metabolism; Tumor Necrosis Factor-alpha - metabolism; tumor necrosis factors
• ISSN: 1436-6207; 1436-6215
• DOI: 10.1007/s00394-012-0482-6

PURPOSE: The dietary sesamol is one of the important constituent of sesame seed that has been mainly claimed to combat cardiovascular disease and diabetes, which are the major secondary complications of arthritis. Thus, the present study was designed to evaluate the anti-arthritic, anti-inflammatory and anti-stress potentials of sesamol. METHODS: Arthritis was induced using Freund’s complete adjuvant to hind paw of experimental rats. The physical and biochemical alterations and its recovery by sesamol were assessed by measuring enzymatic and non-enzymatic mediators. Arthritis-induced inflammation, oxidative stress and their protective by sesamol were measured by determining the levels of pro-inflammatory cytokines and oxidative stress markers. RESULTS: In the present study, sesamol was demonstrated to alleviate arthritis-induced cartilage degeneration by mitigating augmented serum levels of hyaluronidase and matrix metalloproteinases (MMP-13, MMP-3 and MMP-9). It also protected bone resorption by reducing the elevated levels of bone joint exoglycosidases, cathepsin D and tartarate-resistant acid phosphatases. Sesamol also abrogated the non-enzymatic inflammatory markers (TNF, IL-1β, IL-6, COX-2, PGE₂, ROS, and H₂O₂,) effectively. In addition, sesamol neutralizes arthritis-induced oxidative stress by restoring the levels of reactive oxygen species, lipid and hydro peroxides and sustained antioxidant homeostasis by re-establishing altered activities of superoxide dismutase, catalase and glutathione-s-transferase. CONCLUSION: Taken together, the study demonstrated the anti-arthritic, anti-inflammatory, anti-oxidative stress and chondro-protective potentials of sesamol in vivo. Thus, sesamol could be a single bullet that can fight arthritis as well as the secondary complications of arthritis such as cardio vascular disorders and diabetes.