Enhanced Cholesterol Esterification in Monocyte-Derived Macrophages from Diabetic But Not Hypertriglyceridemic Men

To test whether monocyte-derived macrophages obtained from diabetic patients with hypertriglyceridemia have an altered manner of lipid accumulation, we separated monocytes from 7 healthy control subjects, 9 diabetic normolipidemic patients, 9 diabetic hypertriglyceridemic patients, and 7 nondiabetic...

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Bibliographic Details
Published in:Journal of Clinical Biochemistry and Nutrition 1991, Vol.10(1), pp.71-81
Main Authors: HIRAMATSU, Kazuko, NAKANE, Paul K.
Format: Article
Language:English
Subjects:
atherosclerosis
diabetes
human monocyte
hypertriglyceridemia
macrophage
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Summary:To test whether monocyte-derived macrophages obtained from diabetic patients with hypertriglyceridemia have an altered manner of lipid accumulation, we separated monocytes from 7 healthy control subjects, 9 diabetic normolipidemic patients, 9 diabetic hypertriglyceridemic patients, and 7 nondiabetic hypertriglyceridemic patients. The monocytes of each group were modulated to macrophages under two different culture systems using either autologous serum or heterologous normal non-diabetic non-hypertriglyceridemic serum in the culture medium. The cells transformed better in the healthy serum system. In this system, the [14C] oleate incorporation into cholesterol oleate by macrophages derived from the diabetic group (0.30±0.11nmol/mg protein/12h (mean±SD), p
ISSN:0912-0009
1880-5086
DOI:10.3164/jcbn.10.71