Dietary Cholesterol-Induced Hyperlipidemia Modulates Lipid Synthesis in Rabbit Monocytes

• Published in: Journal of Clinical Biochemistry and Nutrition 1993, Vol.15(1), pp.11-21
• Main Authors: GUPTA, Seema, THOMSON, Alan B.R., KAPPAGODA, Tessa, GARG, Manohar
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; cholesterol metabolism; dietary cholesterol; Disorders of blood lipids. Hyperlipoproteinemia; hyperlipidemia; lipid synthesis; Medical sciences; Metabolic diseases; monocytes (rabbit)
• ISSN: 0912-0009; 1880-5086
• DOI: 10.3164/jcbn.15.11

Lipid synthesis in monocytes isolated from rabbits fed a high-cholesterol diet and subsequently a low-cholesterol one was examined. Rabbits were fed diet containing 2.5% (w/w) cholesterol for 3 weeks and then transferred to a normal diet for another 6 weeks. The total and free cholesterol levels in the blood increased up to 3 weeks and then steadily decreased. Esterified cholesterol increased only during the first week on the high-cholesterol diet and then decreased throughout the remainder of the experimental period. Lipid synthesis, as assessed by [1-14C]acetate incorporation, was significantly reduced in monocytes isolated from animals on the high-cholesterol diet. This reduction in lipid synthetic activity continued another 3 weeks even after cessation of the high-cholesterol diet and then lipid synthesis was rather increased significantly. Specifically an increase in cholesterol and fatty acid synthesis was observed when animals were fed a high-cholesterol diet for 3 weeks and then allowed to recover for another 6 weeks.