Structural characterization and antioxidant properties of polysaccharides from two Schisandra fruits

Different polysaccharides (NPS1-1 and BPS1-1) were obtained from Schisandra sphenanthera and Schisandra chinensis fruits by hot-water extraction, DEAE-52 ion-exchange column chromatography and Sephadex G-150 gel filtration chromatography. Structure characteristic and antioxidant properties of NPS1-1...

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Bibliographic Details
Published in:European food research & technology 2013-11, Vol.237 (5), p.691-701
Main Authors: Chen, Shuhan, Chen, Haixia, Wang, Zhaoshuai, Tian, Jingge, Wang, Jia
Format: Article
Language:English
Subjects:
Agriculture
Analytical Chemistry
Antioxidants
Biotechnology
Chemistry
Chemistry and Materials Science
Chromatography
Ethanol
Food
Food Science
Forestry
Fourier transforms
Fruits
Gas chromatography
Glucose
Infrared spectroscopy
Light scattering
Lipid peroxidation
Molecular weight
NMR
Nuclear magnetic resonance
Original Paper
Peroxidation
Saccharides
Scanning electron microscopy
Spectrum analysis
Statistical analysis
Viscosity
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Summary:Different polysaccharides (NPS1-1 and BPS1-1) were obtained from Schisandra sphenanthera and Schisandra chinensis fruits by hot-water extraction, DEAE-52 ion-exchange column chromatography and Sephadex G-150 gel filtration chromatography. Structure characteristic and antioxidant properties of NPS1-1 and BPS1-1 were comparatively investigated by gas chromatography, Fourier transform infrared spectroscopy, scanning electron microscopy, multi-angle laser light scattering analysis, atomic force microscope, circular dichroism spectroscopy, scavenging DPPH free radicals assay, ferric-reducing power assay and lipid peroxidation inhibition assay, respectively. Results showed that physicochemical and antioxidant properties of NPS1-1 and BPS1-1 were different from each other. Molecular weights of NPS1-1 and BPS1-1 were 2.22 × 10 6 and 2.66 × 10 6 , respectively. NPS1-1 and BPS1-1 could be a kind of rhamnogalacturonan, and the sugar chain contained pyran configurations and β-glycosidic linkages. Significant differences in the surface features and particle distribution of NPS1-1 and BPS1-1 were detected. NPS1-1 showed higher antioxidant abilities on ferric-reducing power and lipid peroxidation inhibition activity. The results would be helpful for the further use of the two fruits.
ISSN:1438-2377
1438-2385
DOI:10.1007/s00217-013-2044-4