in vitro mutagenesis protocol for the production of sugarcane tolerant to the herbicide imazapyr

A protocol was developed to induce and identify imazapyr tolerance in sugarcane, which involved induction of somaclonal variation via exposure to 8 or 16 mM ethyl methanesulfonate for 4 h, followed by a stepwise increase in imazapyr concentration in the medium from 0.08 to 0.16 μM. The regenerated p...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Plant 2012-08, Vol.48 (4), p.417-427
Main Authors: Koch, Aimée C, Ramgareeb, Sumita, Rutherford, R. Stuart, Snyman, Sandra J, Watt, M. Paula
Format: Article
Language:English
Subjects:
aboveground biomass
Acclimatization
acetolactate synthase
Biomedical and Life Sciences
Biosynthesis
Callus
Cell Biology
Crops
Cultivars
Deoxyribonucleic acid
Developmental Biology
DNA
Enzymes
ethyl methanesulfonate
Genetic mutation
Genotypes
Herbicide resistance
Herbicides
imazapyr
Life Sciences
Mutagenesis
Mutagens
Mutation
Plant Breeding/Biotechnology
Plant Genetics and Genomics
Plant Sciences
Plantlets
Plants
somaclonal variation
SOMATIC CELL GENETICS
Sugar cane
Sugarcane
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Summary:A protocol was developed to induce and identify imazapyr tolerance in sugarcane, which involved induction of somaclonal variation via exposure to 8 or 16 mM ethyl methanesulfonate for 4 h, followed by a stepwise increase in imazapyr concentration in the medium from 0.08 to 0.16 μM. The regenerated plantlets were then acclimatized for 3 mo after which they were sprayed with 182 g a.i. ha−1 imazapyr, and the above-ground biomass was determined after 47 d. Following a 1-mo waiting period for the putative tolerant plants to regrow, acetohydroxyacid synthase (AHAS; EC 2.2.1.6) enzyme assays of the plants that survived and showed a normal growth pattern were undertaken. Based on the enzymatic I 50 values, three imazapyr-tolerant genotypes were identified with an AHAS activity of 2.8 to 4.0 times that in sensitive sugarcane plants.
ISSN:1054-5476
1475-2689
DOI:10.1007/s11627-012-9448-x