Process of aerenchyma formation and reactive oxygen species induced by waterlogging in wheat seminal roots

• Published in: Planta 2013-11, Vol.238 (5), p.969-982
• Main Authors: Xu, Q. T, Yang, L, Zhou, Z. Q, Mei, F. Z, Qu, L. H, Zhou, G. S
• Format: Article
• Language: English
• Subjects: Accumulation; Agriculture; Biomedical and Life Sciences; Cell death; Ecology; enzymes; Epidermal cells; flooded conditions; Fluorescence; Forestry; gene expression; gene expression regulation; Gene Expression Regulation, Plant - drug effects; genes; Genes, Plant - genetics; Homeostasis - drug effects; Homeostasis - genetics; Hydrogen peroxide; Life Sciences; Meristem - cytology; Meristem - metabolism; Models, Biological; Mortality; Original Article; Oxygen; Plant cells; Plant roots; Plant Roots - cytology; Plant Roots - drug effects; Plant Roots - metabolism; Plant Roots - ultrastructure; Plant Sciences; Plants; Reactive oxygen species; Reactive Oxygen Species - metabolism; Root tips; Roots; Seedlings - drug effects; Seedlings - growth & development; Seminal roots; Triticum - cytology; Triticum - genetics; Triticum - metabolism; Triticum - ultrastructure; Water - pharmacology; Waterlogging; Wheat
• ISSN: 0032-0935; 1432-2048
• DOI: 10.1007/s00425-013-1947-4

The development and regulation of aerenchyma in waterlogged conditions were studied in the seminal roots of wheat. Evans blue staining and the first cell death position indicated that the cortical cell death began at the root mid-cortex cells in flooding conditions. Continuous waterlogging treatment caused the spread of cell death from the mid-cortex to the neighboring cells and well-developed aerenchyma was formed after 72 h. Meanwhile, the formation of radial oxygen loss barrier was observed in the exodermis owing to the induction of Casparian bands and lignin deposition. Analysis of aerenchyma along the wheat root revealed that aerenchyma formed at 10 mm from the root tip, significantly increased toward the center of the roots, and decreased toward the basal region of the root. In situ detection of radial oxygen species (ROS) showed that ROS accumulation started in the mid-cortex cells, where cell death began indicating that cell death was probably accompanied by ROS production. Further waterlogging treatments resulted in the accumulation of ROS in the cortical cells, which were the zone for aerenchyma development. Accumulation and distribution of H₂O₂ at the subcellular level were revealed by ultracytochemical localization, which further verified the involvement of ROS in the cortical cell death process (i.e., aerenchyma formation). Furthermore, gene expression analysis indicated that ROS production might be the result of up-regulation of genes encoding for ROS-producing enzymes and the down-regulation of genes encoding for ROS-detoxifying enzymes. These results suggest that aerenchyma development in wheat roots starts in the mid-cortex cells and its formation is regulated by ROS.