Protective Effect of an Extract of Phyllanthus amarus against Radiation-induced Damage in Mice

The radioprotective effect of an extract of the plant Phyllanthus amarus (P. amarus) was investigated in adult BALB/c mice. P. amarus extract (750 mg/kg b. wt and 250 mg/kg b. wt) was administered orally to mice for five days prior to whole body radiation (6 Gy) and for one month after radiation. Th...

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Bibliographic Details
Published in:JOURNAL OF RADIATION RESEARCH 2004, Vol.45 (1), p.133-139
Main Authors: Kumar, K B Hari, Kuttan, Ramadasan
Format: Article
Language:English
Subjects:
Administration, Oral
Animals
BALB 3T3 Cells
Blood Proteins - analysis
Bone Marrow Cells - drug effects
Bone Marrow Cells - pathology
Bone Marrow Cells - radiation effects
Cells, Cultured
Dose-Response Relationship, Drug
Esterases - blood
Female
Leukocyte Count
Mice
Phyllanthus - metabolism
Phytotherapy - methods
Plant Components, Aerial - metabolism
Plant Extracts - administration & dosage
Radiation Injuries, Experimental - pathology
Radiation Injuries, Experimental - prevention & control
Radiation Tolerance - drug effects
Radiation-Protective Agents - administration & dosage
Whole-Body Irradiation
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Summary:The radioprotective effect of an extract of the plant Phyllanthus amarus (P. amarus) was investigated in adult BALB/c mice. P. amarus extract (750 mg/kg b. wt and 250 mg/kg b. wt) was administered orally to mice for five days prior to whole body radiation (6 Gy) and for one month after radiation. The animals were sacrificed on days 3, 9, 12, and 30 after radiation. P. amarus significantly increased the total W.B.C count, bone marrow cellularity, and α-esterase activity as compared to untreated radiation-exposed animals. P. amarus treatment also increased the activity of various antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST), glutathione peroxidase (GPX), and glutathione reductase (GR), both in blood and tissue, which were reduced by radiation treatment. There was also a significant increase in the glutathione (GSH) levels of blood and tissue. Lipid peroxidation levels, which were increased after radiation, were significantly reduced by P. amarus treatment, both in serum and liver. The results collectively indicate that P. amarus extract could increase the antioxidant defense mechanism in mice and there by protect the animals from radiation-induced cellular damage.
ISSN:0449-3060
1349-9157
DOI:10.1269/jrr.45.133