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Targeted dietary micronutrient fortification modulates n−3 LC-PUFA pathway activity in rainbow trout (Oncorhynchus mykiss)
Replacing fish oil (FO) in aquafeeds with sustainable alternatives such as vegetable oils (VO) compromises the content of n−3 long-chain polyunsaturated fatty acid (n−3 LC-PUFA) in the edible portions of farmed fish. Endogenous biosynthesis of n−3 LC-PUFA from C18 precursors is catalysed by several...
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Published in: | Aquaculture 2013-11, Vol.412-413, p.215-222 |
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creator | Lewis, Michael J. Hamid, Noor Khalidah Abdul Alhazzaa, Ramez Hermon, Karen Donald, John A. Sinclair, Andrew J. Turchini, Giovanni M. |
description | Replacing fish oil (FO) in aquafeeds with sustainable alternatives such as vegetable oils (VO) compromises the content of n−3 long-chain polyunsaturated fatty acid (n−3 LC-PUFA) in the edible portions of farmed fish. Endogenous biosynthesis of n−3 LC-PUFA from C18 precursors is catalysed by several enzymes, which have low activity in carnivorous fish. Rainbow trout were fed on VO-based diets supplemented with increasing levels of selected micronutrients as potential n−3 LC-PUFA biosynthesis co-factors or coenzyme precursors: iron, zinc, magnesium, niacin, riboflavin, pyridoxine and biotin at 100, 200, 300 or 400% of their recommended dietary inclusion. Providing the substrate (ALA, 18:3n−3) and the potential enzyme co-factors was assumed to enhance the efficiency of EPA (20:5n−3) and DHA (22:6n−3) production. Initial evidence was established when DHA and total n−3 LC-PUFA content increased in the whole body of fish from the treatment with the highest micronutrient fortification. Fewer changes were observed in the fillet or liver which was consistent with a marginal regulation of the mRNA expression of key biosynthesis genes in the liver. The potential co-factors seem to stimulate the n−3 LC-PUFA biosynthesis efficiency at the molecular and enzymatic level in rainbow trout fed on ALA-rich diet, leading to metabolic and chemical changes. The interactions between dietary substrate and enzyme co-factors/coenzymes need to be further investigated to advance lipid metabolism research and benefit the aquaculture industry.
•Vitamins (B2, B3, B6, B7) and minerals (Fe, Zn, Mg) are involved in LC-PUFA production.•Rainbow trout were fed on vegetable oil with increasing levels of these micronutrients.•DHA and total n−3 LC-PUFA increased in the whole body but not the fillet or liver.•The micronutrients modulated mRNA transcription of key enzymes in the liver.•There is an evidence on enhancing n−3 LC-PUFA production from ALA by micronutrient fortification. |
doi_str_mv | 10.1016/j.aquaculture.2013.07.024 |
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•Vitamins (B2, B3, B6, B7) and minerals (Fe, Zn, Mg) are involved in LC-PUFA production.•Rainbow trout were fed on vegetable oil with increasing levels of these micronutrients.•DHA and total n−3 LC-PUFA increased in the whole body but not the fillet or liver.•The micronutrients modulated mRNA transcription of key enzymes in the liver.•There is an evidence on enhancing n−3 LC-PUFA production from ALA by micronutrient fortification.</description><identifier>ISSN: 0044-8486</identifier><identifier>EISSN: 1873-5622</identifier><identifier>DOI: 10.1016/j.aquaculture.2013.07.024</identifier><identifier>CODEN: AQCLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animal and plant ecology ; Animal aquaculture ; Animal productions ; Animal, plant and microbial ecology ; Aquaculture ; Autoecology ; Biological and medical sciences ; Biosynthesis ; Coenzymes ; Cofactors ; Diet ; Fatty acid ; Fish oil replacement ; Fundamental and applied biological sciences. Psychology ; General aspects ; Minerals ; Trout ; Vitamins</subject><ispartof>Aquaculture, 2013-11, Vol.412-413, p.215-222</ispartof><rights>2013 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright Elsevier Sequoia S.A. Nov 1, 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c445t-ce483c1f20e07592747507ec9090d9671cbfa3eef7a7589e9c8c8e1dff6d6f9d3</citedby><cites>FETCH-LOGICAL-c445t-ce483c1f20e07592747507ec9090d9671cbfa3eef7a7589e9c8c8e1dff6d6f9d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27938978$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Lewis, Michael J.</creatorcontrib><creatorcontrib>Hamid, Noor Khalidah Abdul</creatorcontrib><creatorcontrib>Alhazzaa, Ramez</creatorcontrib><creatorcontrib>Hermon, Karen</creatorcontrib><creatorcontrib>Donald, John A.</creatorcontrib><creatorcontrib>Sinclair, Andrew J.</creatorcontrib><creatorcontrib>Turchini, Giovanni M.</creatorcontrib><title>Targeted dietary micronutrient fortification modulates n−3 LC-PUFA pathway activity in rainbow trout (Oncorhynchus mykiss)</title><title>Aquaculture</title><description>Replacing fish oil (FO) in aquafeeds with sustainable alternatives such as vegetable oils (VO) compromises the content of n−3 long-chain polyunsaturated fatty acid (n−3 LC-PUFA) in the edible portions of farmed fish. Endogenous biosynthesis of n−3 LC-PUFA from C18 precursors is catalysed by several enzymes, which have low activity in carnivorous fish. Rainbow trout were fed on VO-based diets supplemented with increasing levels of selected micronutrients as potential n−3 LC-PUFA biosynthesis co-factors or coenzyme precursors: iron, zinc, magnesium, niacin, riboflavin, pyridoxine and biotin at 100, 200, 300 or 400% of their recommended dietary inclusion. Providing the substrate (ALA, 18:3n−3) and the potential enzyme co-factors was assumed to enhance the efficiency of EPA (20:5n−3) and DHA (22:6n−3) production. Initial evidence was established when DHA and total n−3 LC-PUFA content increased in the whole body of fish from the treatment with the highest micronutrient fortification. Fewer changes were observed in the fillet or liver which was consistent with a marginal regulation of the mRNA expression of key biosynthesis genes in the liver. The potential co-factors seem to stimulate the n−3 LC-PUFA biosynthesis efficiency at the molecular and enzymatic level in rainbow trout fed on ALA-rich diet, leading to metabolic and chemical changes. The interactions between dietary substrate and enzyme co-factors/coenzymes need to be further investigated to advance lipid metabolism research and benefit the aquaculture industry.
•Vitamins (B2, B3, B6, B7) and minerals (Fe, Zn, Mg) are involved in LC-PUFA production.•Rainbow trout were fed on vegetable oil with increasing levels of these micronutrients.•DHA and total n−3 LC-PUFA increased in the whole body but not the fillet or liver.•The micronutrients modulated mRNA transcription of key enzymes in the liver.•There is an evidence on enhancing n−3 LC-PUFA production from ALA by micronutrient fortification.</description><subject>Animal and plant ecology</subject><subject>Animal aquaculture</subject><subject>Animal productions</subject><subject>Animal, plant and microbial ecology</subject><subject>Aquaculture</subject><subject>Autoecology</subject><subject>Biological and medical sciences</subject><subject>Biosynthesis</subject><subject>Coenzymes</subject><subject>Cofactors</subject><subject>Diet</subject><subject>Fatty acid</subject><subject>Fish oil replacement</subject><subject>Fundamental and applied biological sciences. 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Endogenous biosynthesis of n−3 LC-PUFA from C18 precursors is catalysed by several enzymes, which have low activity in carnivorous fish. Rainbow trout were fed on VO-based diets supplemented with increasing levels of selected micronutrients as potential n−3 LC-PUFA biosynthesis co-factors or coenzyme precursors: iron, zinc, magnesium, niacin, riboflavin, pyridoxine and biotin at 100, 200, 300 or 400% of their recommended dietary inclusion. Providing the substrate (ALA, 18:3n−3) and the potential enzyme co-factors was assumed to enhance the efficiency of EPA (20:5n−3) and DHA (22:6n−3) production. Initial evidence was established when DHA and total n−3 LC-PUFA content increased in the whole body of fish from the treatment with the highest micronutrient fortification. Fewer changes were observed in the fillet or liver which was consistent with a marginal regulation of the mRNA expression of key biosynthesis genes in the liver. The potential co-factors seem to stimulate the n−3 LC-PUFA biosynthesis efficiency at the molecular and enzymatic level in rainbow trout fed on ALA-rich diet, leading to metabolic and chemical changes. The interactions between dietary substrate and enzyme co-factors/coenzymes need to be further investigated to advance lipid metabolism research and benefit the aquaculture industry.
•Vitamins (B2, B3, B6, B7) and minerals (Fe, Zn, Mg) are involved in LC-PUFA production.•Rainbow trout were fed on vegetable oil with increasing levels of these micronutrients.•DHA and total n−3 LC-PUFA increased in the whole body but not the fillet or liver.•The micronutrients modulated mRNA transcription of key enzymes in the liver.•There is an evidence on enhancing n−3 LC-PUFA production from ALA by micronutrient fortification.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><doi>10.1016/j.aquaculture.2013.07.024</doi><tpages>8</tpages></addata></record> |
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subjects | Animal and plant ecology Animal aquaculture Animal productions Animal, plant and microbial ecology Aquaculture Autoecology Biological and medical sciences Biosynthesis Coenzymes Cofactors Diet Fatty acid Fish oil replacement Fundamental and applied biological sciences. Psychology General aspects Minerals Trout Vitamins |
title | Targeted dietary micronutrient fortification modulates n−3 LC-PUFA pathway activity in rainbow trout (Oncorhynchus mykiss) |
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