Discrimination of Isomeric Fragment Ions Observed in Tandem Mass Spectra of Biantennary Oligosaccharides by Use of Selective Isotope Labeling

We report herein the first mass spectrometric analyses of isotopomers of a pyridylamino (PA)-derivatized biantennary oligosaccharide, in which one of two non-reducing terminal galactose residues is selectively enriched in 13C by an enzymatic reaction. By use of these isotopomeric PA-oligosaccharides...

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Bibliographic Details
Published in:Journal of the Mass Spectrometry Society of Japan 2004, Vol.52(5), pp.284-288
Main Authors: KATO, Koichi, YAMAGUCHI, Yoshiki, TAKAHASHI, Noriko, NISHIMURA, Mamiko, IWAMOTO, Shin-ichi, SEKIYA, Sadanori, TANAKA, Koichi
Format: Article
Language:English
Subjects:
Ion trap quadrupole
Isomers
Isotope
Oligosaccharides
Tandem mass spectra
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Summary:We report herein the first mass spectrometric analyses of isotopomers of a pyridylamino (PA)-derivatized biantennary oligosaccharide, in which one of two non-reducing terminal galactose residues is selectively enriched in 13C by an enzymatic reaction. By use of these isotopomeric PA-oligosaccharides, individual fragment ions observed in tandem mass spectra of MALDI-QIT-TOF-MS were unambiguously assigned. It was shown that the oligosaccharide ion that lacks the N-acetyllactosamine previously linked to the Manα1 →6 antenna dominates its isomeric counterpart ion. We propose that tandem mass spectrometric analyses of oligosaccharides isotopically labeled at a selected antenna would provide us with the basis of mechanisms of formation of glycosidic linkage fragment ions.
ISSN:1340-8097
1880-4225
DOI:10.5702/massspec.52.284