Enantioselective Radioimmunoassay for (±)-5(Z)-7-(3-endo- Phenylsulfonylamino-bicyclohept[2.2.1]-exo-2-yl)heptenoic Acid, a Thromboxane A2 Receptor Antagonist

A method has been developed for the enantioselective determination of the title compound S-145, a novel thromboxane A2 receptor antagonist, in a plasma by competitive radioimmunoassay. In order to obtain an antiserum that is optically specific to (+)-S-145 or (-)-S-145, hapten-carrier conjugates wer...

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Bibliographic Details
Published in:Analytical Sciences 1991/02/10, Vol.7(1), pp.33-37
Main Authors: MURAI, Yasuo, MORI, Shusuke, KOMINAMI, Goro, KONO, Masao
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Enantioselectivity
Fundamental and applied biological sciences. Psychology
optical isomer
Other biological molecules
plasma level
radioimmunoassay
thromboxane receptor antagonist
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Summary:A method has been developed for the enantioselective determination of the title compound S-145, a novel thromboxane A2 receptor antagonist, in a plasma by competitive radioimmunoassay. In order to obtain an antiserum that is optically specific to (+)-S-145 or (-)-S-145, hapten-carrier conjugates were individually prepared by coupling with bovine serum albumin. In displacement studies with enantiomerically pure radioligands, the opposite enantiomer showed a negligible cross-reaction (0.1 to 0.3%) and the racemate showed about a 50% cross-reaction. No cross- reactivity was found with related prostanoids, except for a few metabolites of S-145. The minimum detectable dose (p
ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.7.33