Time-Resolved Immunofluorometric Assay for Human Interferon-[gamma]

A time-resolved Immunofluorometric assay (TR-IFMA) for interferon-γ (IFN-γ) was developed and its properties were compared with those of previously reported immunoenzymometric assays (IEMAs). The advantage of TR-IFMA is not only that it is as sensitive as the usual colorimetric IFMA, but that it is...

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Bibliographic Details
Published in:Analytical sciences 1995-05, Vol.11 (3), p.411
Main Authors: HARA, Hirokazu, CHIKUMA, Masahiko, KOMINAMI, Goro
Format: Article
Language:English
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Summary:A time-resolved Immunofluorometric assay (TR-IFMA) for interferon-γ (IFN-γ) was developed and its properties were compared with those of previously reported immunoenzymometric assays (IEMAs). The advantage of TR-IFMA is not only that it is as sensitive as the usual colorimetric IFMA, but that it is also feasible and fast as a detection procedure of europium. It was modified in order to make it more sensitive and faster by changing the antiserum and incubation conditions. The improved assay could directly measure IFN-γ in human plasma without any pretreatment. The detection limit for IFN-γ is 40pg/ml, and an assay can be completed within 6 h.
ISSN:0910-6340
1348-2246