Highly Selective Needle-Type Glucose Sensors Prepared by the Immobilization of Glucose Oxidase on [gamma]-Polyglutamic Acid Film

Fine needle-type glucose sensors with an outer diameter of less that 0.2 mm were fabricated using a low-cost biodegradable γ-polyglutamic acid (PGA) not only as a glucose oxidase (GOx) immobilizing material, but also as a permselective inner membrane. PGA film was prepared on a cellulose acetate-coa...

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Bibliographic Details
Published in:Analytical sciences 2011-03, Vol.27 (3)
Main Authors: YASUZAWA, Mikito, EDAGAWA, Kazuaki, MATSUNAGA, Takuya, TAKAOKA, Hiroki, YABUTANI, Tomoki
Format: Article
Language:English
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Summary:Fine needle-type glucose sensors with an outer diameter of less that 0.2 mm were fabricated using a low-cost biodegradable γ-polyglutamic acid (PGA) not only as a glucose oxidase (GOx) immobilizing material, but also as a permselective inner membrane. PGA film was prepared on a cellulose acetate-coated Pt-Ir wire electrode, and GOx was covalently immobilized on its surface using water-soluble carbodiimide. The obtained electrode was practically not affected by the existence of electroactive compounds, and provided long-term stability for approximately one month. It also functioned in horse serum with a good linear relationship between the current and glucose concentrations within the range of 2.8 to 22.4 mM.
ISSN:0910-6340
1348-2246