Detection of metallo-beta-lactamase in Pseudomonas aeruginosa isolated from hospitalized patients in Goiânia, State of Goiás

• Published in: Revista da Sociedade Brasileira de Medicina Tropical 2009-07, Vol.42 (4), p.411
• Main Authors: Gonçalves, Diana Christina Pereira Santos, Lima, Ana Beatriz Mori, Leão, Lara Stefania Netto de Oliveira, Filho, José Rodrigues do Carmo, Pimenta, Fabiana Cristina, Vieira, José Daniel Gonçalves
• Format: Article
• Language: eng ; por
• Subjects: Anti-Bacterial Agents - pharmacology; beta-Lactamases - biosynthesis; beta-Lactamases - genetics; Brazil; Cross Infection - microbiology; Disk Diffusion Antimicrobial Tests; Drug Resistance, Multiple, Bacterial - genetics; Humans; Phenotype; Polymerase Chain Reaction; Pseudomonas aeruginosa - drug effects; Pseudomonas aeruginosa - enzymology; Pseudomonas aeruginosa - genetics; Pseudomonas Infections - microbiology
• ISSN: 0037-8682; 1678-9849
• DOI: 10.1590/S0037-86822009000400010

Pseudomonas aeruginosa is a bacterium frequently isolated from hospital environments. This study had the aims of evaluating the susceptibility profile of Pseudomonas aeruginosa previously isolated from patients in a hospital in Goiânia (Goiás, Brazil), performing phenotypic screening for metallo-beta-lactamase production and detecting its genes using the polymerase chain reaction technique. Seventy-five 75 Pseudomonas aeruginosa isolates were evaluated between January 2005 and January 2007. Biochemical identification was performed using the API 20E system and an antibiogram was produced using the Kirby-Bauer method. Among the 62 isolates that were resistant to imipenem and ceftazidime, 35 (56.4%) produced metallo-beta-lactamase, while 26 (74.3%) showed the bla(SPM-1) gene. The frequency of Pseudomonas aeruginosa that produces metallo-beta-lactamase suggests that greater control over the dissemination of resistance in hospital environments is needed.