Detection of metallo-beta-lactamase in Pseudomonas aeruginosa isolated from hospitalized patients in Goiânia, State of Goiás

Pseudomonas aeruginosa is a bacterium frequently isolated from hospital environments. This study had the aims of evaluating the susceptibility profile of Pseudomonas aeruginosa previously isolated from patients in a hospital in Goiânia (Goiás, Brazil), performing phenotypic screening for metallo-bet...

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Published in:Revista da Sociedade Brasileira de Medicina Tropical 2009-07, Vol.42 (4), p.411
Main Authors: Gonçalves, Diana Christina Pereira Santos, Lima, Ana Beatriz Mori, Leão, Lara Stefania Netto de Oliveira, Filho, José Rodrigues do Carmo, Pimenta, Fabiana Cristina, Vieira, José Daniel Gonçalves
Format: Article
Language:eng ; por
Subjects:
Anti-Bacterial Agents - pharmacology
beta-Lactamases - biosynthesis
beta-Lactamases - genetics
Brazil
Cross Infection - microbiology
Disk Diffusion Antimicrobial Tests
Drug Resistance, Multiple, Bacterial - genetics
Humans
Phenotype
Polymerase Chain Reaction
Pseudomonas aeruginosa - drug effects
Pseudomonas aeruginosa - enzymology
Pseudomonas aeruginosa - genetics
Pseudomonas Infections - microbiology
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container_title Revista da Sociedade Brasileira de Medicina Tropical
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creator Gonçalves, Diana Christina Pereira Santos
Lima, Ana Beatriz Mori
Leão, Lara Stefania Netto de Oliveira
Filho, José Rodrigues do Carmo
Pimenta, Fabiana Cristina
Vieira, José Daniel Gonçalves
description Pseudomonas aeruginosa is a bacterium frequently isolated from hospital environments. This study had the aims of evaluating the susceptibility profile of Pseudomonas aeruginosa previously isolated from patients in a hospital in Goiânia (Goiás, Brazil), performing phenotypic screening for metallo-beta-lactamase production and detecting its genes using the polymerase chain reaction technique. Seventy-five 75 Pseudomonas aeruginosa isolates were evaluated between January 2005 and January 2007. Biochemical identification was performed using the API 20E system and an antibiogram was produced using the Kirby-Bauer method. Among the 62 isolates that were resistant to imipenem and ceftazidime, 35 (56.4%) produced metallo-beta-lactamase, while 26 (74.3%) showed the bla(SPM-1) gene. The frequency of Pseudomonas aeruginosa that produces metallo-beta-lactamase suggests that greater control over the dissemination of resistance in hospital environments is needed.
doi_str_mv 10.1590/S0037-86822009000400010
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subjects Anti-Bacterial Agents - pharmacology
beta-Lactamases - biosynthesis
beta-Lactamases - genetics
Brazil
Cross Infection - microbiology
Disk Diffusion Antimicrobial Tests
Drug Resistance, Multiple, Bacterial - genetics
Humans
Phenotype
Polymerase Chain Reaction
Pseudomonas aeruginosa - drug effects
Pseudomonas aeruginosa - enzymology
Pseudomonas aeruginosa - genetics
Pseudomonas Infections - microbiology
title Detection of metallo-beta-lactamase in Pseudomonas aeruginosa isolated from hospitalized patients in Goiânia, State of Goiás
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