Studies on Thermophile Products. X. Further Biological Properties of Isofatty Acid-Containing Phosphatidylglycerol That Enhances the Induction of Suppressor T Cells

• Published in: Biological & pharmaceutical bulletin 1994/11/15, Vol.17(11), pp.1446-1450
• Main Authors: KOHAMA, Yasuhiro, SEMBA, Taro, TANAKA, Keishi, IIDA, Kentaro, MORIYAMA, Kazuki, SUDA, Toshio, TONE, Hideki, ITOH, Susumu, MIMURA, Tsutomu
• Format: Article
• Language: English
• Subjects: Animals; Bacillus stearothermophilus; Biological and medical sciences; Cells, Cultured; Concanavalin A - toxicity; Dinoprostone - metabolism; Dose-Response Relationship, Drug; Fatty Acids - chemistry; Geobacillus stearothermophilus - metabolism; Immunomodulators; Indomethacin - pharmacology; isofatty acid; Lymphocyte Activation - drug effects; Lymphocyte Culture Test, Mixed; Major Histocompatibility Complex - drug effects; Major Histocompatibility Complex - immunology; Male; Medical sciences; Mice; Mice, Inbred BALB C; mixed lymphocyte reaction; Pharmacology. Drug treatments; phosphatidylglycerol; Phosphatidylglycerols - chemistry; Phosphatidylglycerols - isolation & purification; Phosphatidylglycerols - pharmacology; prostaglandin E2; Spleen - cytology; Spleen - drug effects; suppressor T cell; T-Lymphocytes, Regulatory - drug effects; T-Lymphocytes, Regulatory - immunology
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.17.1446

Isofatty acid-containing phosphatidylglycerol (Fr. 7-C), isolated from Bacillus stearothermophilus UBT8038, enhances the induction of concanavalin A (Con A)-activated suppressor T (Ts) cells in a dose dependent manner (0.01-1μg/ml). Its further biological properties on mouse mixed lymphocyte reaction (MLR) has been demonstrated. Fr. 7-C (0.01-1 μg/ml) suppressed the MLR at 4 d in a dose-dependent manner when added at the start of splenocyte cultivation. Moreover, Fr. 7-C was effective in preventing the generation of cytotoxic T lymphocytes after the MLR. On the other hand, this fraction significantly enhanced the induction of Ts cells in the MLR carried out in any of the antigen-specific, antigen-nonspecific and major histocompatibility complex antigen-nonrestricted fashions. Fr. 7-C increased prostaglandin E2 (PGE2) release approximately 2-fold in the culture supernatant of Con A-activated splenocytes, and PGE2 release decreased dose-dependently when cultured with indomethacin. The inhibitory effect by Fr. 7-C on the MLR was abrogated by the addition of indomethacin. The enhancement by Fr. 7-C on Ts cell induction was blocked by indomethacin in a dose dependent manner. These results strongly suggest that Fr. 7-C suppresses the MLR via the enhancement of antigen-nonspecific Ts cell induction mediated at least partly by PGE2.