Biological and histopathological characterization together with nucleic acids analysis of a Trypanosoma cruzi strain from Marília, São Paulo State

• Published in: Revista da Sociedade Brasileira de Medicina Tropical 2003-01, Vol.36 (1), p.35
• Main Authors: Martins, Luciamáre Perinetti Alves, Castanho, Roberto Esteves Pires, da Rosa, João Aristeu, da Silva, Luiz Carlos, de Godoy, Charles Antônio Pires, Rosa, Raphael de Maurício
• Format: Article
• Language: eng ; por
• Subjects: Animals; Deoxyribonucleic acid; DNA; DNA, Protozoan - analysis; Humans; Male; Mice; Middle Aged; Nucleic acids; Parasitemia - parasitology; Polymerase Chain Reaction; Trypanosoma cruzi - genetics; Trypanosoma cruzi - pathogenicity
• ISSN: 0037-8682; 1678-9849
• DOI: 10.1590/S0037-86822003000100006

The aim of this report was to study the biological and histopathological behavior of a Trypanosoma cruzi strain, which is found in the region of Marília. The strain was isolated in 1997, by artificial xenodiagnosis. Twenty-five swiss mice were intraperitoneally inoculated. Eleven were used for observation of parasitemia and trypomastigotes morphology and 14 were sacrificed after 17, 23, 30, 60 and 180 days post-infection. Heart, esophagus, liver, colon, and skeletal muscle (fragment of the right thigh) were collected for histopathological study. LIT culture medium was accomplished for DNA analysis. The results showed predominance of broad forms, low parasitemia with mean peaks of 860 trypomastigotes/5mul of blood of the 20th day of infection. No animal died in the acute phase of infection. Histopathological analysis showed several pseudocysts of amastigotes in heart, rare in skeletal muscle and colon with discreet inflammatory process. When Famema strain was compared with Y strain, which was isolated from a patient who lived in the same area, a distinct behaviour and biological characteristics were observed. However, DNA analysis placed them in same group, hence displaying the proximity of these strains.