Butein Ameliorates Renal Concentrating Ability in Cisplatin-Induced Acute Renal Failure in Rats

• Published in: Biological & Pharmaceutical Bulletin 2004, Vol.27(3), pp.366-370
• Main Authors: Kang, Dae Gill, Lee, An Sook, Mun, Yeun Ja, Woo, Won Hong, Kim, Youn Chul, Sohn, Eun Jin, Moon, Mi Kyong, Lee, Ho Sub
• Format: Article
• Language: English
• Subjects: Acute Kidney Injury - chemically induced; Acute Kidney Injury - pathology; Acute Kidney Injury - prevention & control; Animals; Antineoplastic Agents - toxicity; Aquaporin 2; Aquaporins - biosynthesis; Blotting, Western; butein; Chalcone - analogs & derivatives; Chalcone - pharmacology; Chalcone - therapeutic use; Chalcones; Chlorides - urine; Cisplatin - toxicity; cisplatin-nephrotoxicity; Creatinine - blood; Kidney Function Tests; Kidney Medulla - metabolism; Male; Potassium - urine; Rats; Rats, Sprague-Dawley; renal concentrating ability; Sodium - urine
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.27.366

The present study examined whether the cisplatin-induced nephropathy could be ameliorated by administration of butein isolated from the stems of Rhus verniciflua STOCKS. The present study showed that polyuric profile was revealed in cisplatin-induced acute renal failure (ARF) rats associated with decreases in urinary sodium, potassium, chloride, and creatinine excretion, and osmolality. Among these renal functional parameters, urinary volume and osmolality were partially restored by administration of butein (10 mg/kg, i.p.), but electrolytes and creatinine excretion were not restored. Both solute-free water reabsorption and creatinine clearance were also significantly decreased in rats subjected to cisplatin. When butein was administered in rats with cisplatin-induced ARF for 4 d, solute-free water reabsorption was improved by 91% compared with that of cisplatin-induced ARF rats, but creatinine clearance was not restored. The expression levels of aquaporin 2 (AQP 2) in the inner, outer medulla, and cortex were significantly decreased in the kidney of ARF, which were partially reverted by administration of butein. In histological examination of the kidney, butein treatment partially prevented the lesions at tubules of renal cortex in cisplatin-induced ARF rats, while the lesions at glomeruli were not ameliorated. Taken together, butein ameliorates renal concentrating ability via up-regulation of renal AQP 2 water channel in rats with cisplatin-induced ARF without ameliorating effect on renal filtration defect.