Protective Effects of Panduratin A against Oxidative Damage of tert-Butylhydroperoxide in Human HepG2 Cells

• Published in: Biological & Pharmaceutical Bulletin 2005, Vol.28(6), pp.1083-1086
• Main Authors: Sohn, Jong Hee, Han, Kyu-Lee, Lee, Sun-Hee, Hwang, Jae-Kwan
• Format: Article
• Language: English
• Subjects: Antioxidants - isolation & purification; Antioxidants - pharmacology; Cell Line, Tumor; Chalcones; HepG2 cell; Humans; oxidative damage; Oxidative Stress - drug effects; Oxidative Stress - physiology; panduratin A; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Rhizome; tert-butylhydroperoxide; tert-Butylhydroperoxide - antagonists & inhibitors; tert-Butylhydroperoxide - toxicity; Zingiberaceae
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.28.1083

The protective effect of panduratin A, isolated from Kaempferia pandurata ROXB. (Zingiberaceae), against tert-butylhydroperoxide (t-BHP)-induced cytotoxicity was investigated in a human hepatoma cell line, HepG2. The tetrazolium dye colorimetric test (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay) was used to monitor cytotoxicity. Lipid peroxidation [malondialdehyde (MDA) formation] and intracellular glutathione level were estimated by fluorometric methods. Intracellular reactive oxygen species (ROS) formation was measured using a fluorescent probe 2′,7′-dichlorofluorescein diacetate (DCFH-DA). Panduratin A significantly reduced the cell growth inhibition caused by t-BHP. Furthermore, panduratin A ameliorated lipid peroxidation as demonstrated by a reduction in MDA formation, and attenuated glutathione (GSH) depletion in a dose-dependent manner. It was also found that panduratin A reduced intracellular ROS formation caused by t-BHP. These results strongly suggest that panduratin A has significant protective ability against oxidative damage caused by reactive intermediates.