Gentamicin-Induced Nephrotoxicity in Rats Ameliorated and Healing Effects of Resveratrol

• Published in: Biological & Pharmaceutical Bulletin 2007, Vol.30(1), pp.79-83
• Main Authors: Silan, Coşkun, Uzun, Özge, Çomunoglu, Nil Üstündag, Gokçen, Sanem, Bedirhan, Selma, Cengiz, Müjgan
• Format: Article
• Language: English
• Subjects: Animals; Anti-Bacterial Agents; antioxidant; Antioxidants - pharmacology; Antioxidants - therapeutic use; Catalase - metabolism; Creatinine - blood; gentamicin; Gentamicins; Glutathione - metabolism; Glutathione Transferase - metabolism; Kidney - drug effects; Kidney - metabolism; Kidney - pathology; Kidney Diseases - blood; Kidney Diseases - chemically induced; Kidney Diseases - metabolism; Kidney Diseases - pathology; Kidney Diseases - prevention & control; Kidney Tubules - drug effects; lipid peroxidation; Lipid Peroxidation - drug effects; Male; Malondialdehyde - metabolism; nephrotoxicity; Potassium - blood; Rats; Rats, Wistar; Resveratrol; Sodium - blood; Stilbenes - pharmacology; Stilbenes - therapeutic use; Urea - blood
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.30.79

In this study, we aimed to investigate the possible protective effect of resveratrol on gentamicin induced nephrotoxicity. Experiments were carried out in male Wistar rats weighing 200—250 g. Gentamicin sulfate (80 mg/kg per day i.p.), resveratrol (10 mg/kg per day i.p.) and gentamicin together with resveratrol were administered for 6 d. The animals were sacrificed 24 h after the last injection. Urine, blood samples and tissue samples were collected from the animals on the seventh day of the treatment before they were sacrificed. Kidneys were collected for histopathological studies and fixed in 10% buffered formalin solution. Tissue samples were stored at −70 °C in liquid nitrogen for the determination of glutathione (GSH), glutathione-S-transferase (GST), malondialdehyde (MDA) and catalase (CAT). Glutathione assay was determined by the method of Beutler et al. GST amounts were measured by the method of Habig et al. Catalase activitiy was tested by Aebi's method and MDA was determined according to Thayer's method. Blood urea level was significantly increased in the gentamicin treated group. The study showed lowered levels of urea and creatinine levels in resveratrol administered groups when compared with gentamicin administered rats, and the difference was statistically significant. It has been determined that resveratrol caused statistically significant decrease in lipid peroxidation and reduced the level of catalase. Histopathological examination showed that resveratrol prevented partly gentamicin induced tubular damage. The results histopathologically demonstrated that resveratrol has a protective effect against gentamicin induced nephrotoxicity, lipid peroxidation and cellular damage in rats.