Magnoflorine from Coptidis Rhizoma Protects High Density Lipoprotein during Oxidant Stress

The objective of the present study was to investigate the beneficial properties of magnoflorine, an alkaloid isolated from Coptidis Rhizoma, on protecting human high density lipoprotein (HDL) against lipid peroxidation. Magnoflorine exerts an inhibitory effect against Cu2+-induced lipid peroxidation...

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Bibliographic Details
Published in:Biological & Pharmaceutical Bulletin 2007, Vol.30(6), pp.1157-1160
Main Authors: Hung, Tran Manh, Lee, Jong Pill, Min, Byung Sun, Choi, Jae Sue, Na, MinKyun, Zhang, XinFeng, Ngoc, Tran Minh, Lee, IkSoo, Bae, KiHwan
Format: Article
Language:English
Subjects:
Amidines - toxicity
antioxidant
Antioxidants - chemistry
Antioxidants - pharmacology
Aporphines - chemistry
Aporphines - pharmacology
cardiovascular disease
Copper - toxicity
Coptidis Rhizoma
Dose-Response Relationship, Drug
Drugs, Chinese Herbal - chemistry
high density lipoprotein
Humans
Inhibitory Concentration 50
Lipid Peroxidation - drug effects
Lipoproteins, HDL - metabolism
magnoflorine
Molecular Structure
Oxidative Stress
Thiobarbituric Acid Reactive Substances - analysis
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Summary:The objective of the present study was to investigate the beneficial properties of magnoflorine, an alkaloid isolated from Coptidis Rhizoma, on protecting human high density lipoprotein (HDL) against lipid peroxidation. Magnoflorine exerts an inhibitory effect against Cu2+-induced lipid peroxidation of HDL, as showed by prolongation of lag time from 62 to 123 min at the concentration of 3.0 μM. It also inhibits the generation of thiobarbituric acid reactive substances (TBARS) in the dose-dependent maners with IC50 values of 2.3±0.2 μM and 6.2±0.5 μM since HDL oxidation mediated by either catalytic Cu2+ or thermo-labile radical initiator (AAPH), respectively. Separately, Cu2+ oxidized HDL lost the antioxidant action but the inclusion of magnoflorine/Cu2+ oxidized HDL can protect LDL oxidation according to increasing magnoflorine concentration. The results suggest that magnoflorine may have a role to play in preventing the HDL oxidation.
ISSN:0918-6158
1347-5215
DOI:10.1248/bpb.30.1157