Characterization of a Mutation Responsible for an Alkali-sensitive Mutant, 18224, of Alkaliphilic Bacillus sp. strain C-125

An alkali-sensitive mutant, 18224, of the alkaliphilic Bacillus sp. strain C-125 was characterized. The nucleotide sequence of the PvuI-NlaIV DNA fragment that recovers the alkaliphily of 18224 has been cloned from the mutant and sequenced. Comparison of the nucleotide sequences of the corresponding...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1995, Vol.59 (7), p.1364-1366
Main Authors: Seto, Yasuyuki, Hashimoto, Michizane, Usami, Ron, Hamamoto, Tetsuo, Kudo, Toshiaki, Horikoshi, Koki
Format: Article
Language:English
Subjects:
Alkalies
Amino Acid Sequence
Bacillus
Bacillus - drug effects
Bacillus - genetics
Bacteriology
Base Sequence
Biological and medical sciences
Biotechnology
DNA, Bacterial - analysis
Electrophoresis, Polyacrylamide Gel
Fundamental and applied biological sciences. Psychology
Genetics
Microbiology
Molecular Sequence Data
Mutation
Open Reading Frames
Plasmids
Recombination, Genetic
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Summary:An alkali-sensitive mutant, 18224, of the alkaliphilic Bacillus sp. strain C-125 was characterized. The nucleotide sequence of the PvuI-NlaIV DNA fragment that recovers the alkaliphily of 18224 has been cloned from the mutant and sequenced. Comparison of the nucleotide sequences of the corresponding regions found a G to A substitution in the mutant. The mutation resulted in an amino acid substitution from 82 Gly to Glu of the putative ORF3 product, which consisted a gene cluster of at least four tandemly located open reading frames. The ORF3 product was deduced to be an 112 amino acid polypeptide with hydrophobic properties, which was expressed using an in vitro translation system.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.59.1364