Enzymatic Synthesis of 4-O-[beta]-D-Galactopyranosyl-moranoline and 3-O-[beta]-D-Galacto-pyranosyl-moranoline by Using [beta]-Galactosidase from Bacillus circulans

A transgalactosylation reaction from lactose to moranoline (1-deoxynojirimycin) was accomplished by using β-galactosidase [EC 3.2.1.23] from Bacillus circulans. The enzyme formed 3-O-β-D-galactopyranosyl-moranoline and 4-O-β-D-galactopyranosyl-mor-anolinie as major products, together with 2-O-β-D-ga...

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Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1996-04, Vol.60 (4), p.694
Main Authors: KOJIMA, Masahiko, SETO, Takashi, KYOTANI, Yohsuke, OGAWA, Hirotsugu, KITAZAWA, Satoru, MORI, Kazuya, MARUO, Shigeaki, OHGI, Tadaaki, EZURE, Yohji
Format: Article
Language:English
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Summary:A transgalactosylation reaction from lactose to moranoline (1-deoxynojirimycin) was accomplished by using β-galactosidase [EC 3.2.1.23] from Bacillus circulans. The enzyme formed 3-O-β-D-galactopyranosyl-moranoline and 4-O-β-D-galactopyranosyl-mor-anolinie as major products, together with 2-O-β-D-galactopranosyl-moranoline and 6-O-β-D-galactopranosyl-moranoline as minor ones.
ISSN:0916-8451
1347-6947