A Novel Proteinaceous Kex 2 Proteinase Inhibitor, Kexstatin, from Streptomyces platensis Q268

We found a novel proteinaceous Kex 2 proteinase inhibitor, named kexstatin, in the culture supernatant of Streptomyces platensis Q268. The purified kexstatin was homogeneous by SDS-PAGE and the molecular weight was estimated to be 13,000. The N-terminal amino acid sequence of kexstatin has high simi...

Full description

Saved in:
Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1996, Vol.60 (8), p.1388-1389
Main Authors: Oda, Kohei, Takahashi, Saori, Kikuchi, Norihisa, Shibano, Yuji
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Analytical, structural and metabolic biochemistry
Bacterial Proteins - isolation & purification
Biological and medical sciences
Biotechnology
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
Hydrolases
inhibitors
Kex 2 proteinase
Molecular Sequence Data
Proprotein Convertases
Protease Inhibitors - isolation & purification
Saccharomyces cerevisiae Proteins
Sequence Homology, Amino Acid
SSI
Streptomyces
Streptomyces - chemistry
Streptomyces platensis
subtilisin
Subtilisins - antagonists & inhibitors
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We found a novel proteinaceous Kex 2 proteinase inhibitor, named kexstatin, in the culture supernatant of Streptomyces platensis Q268. The purified kexstatin was homogeneous by SDS-PAGE and the molecular weight was estimated to be 13,000. The N-terminal amino acid sequence of kexstatin has high similarity to Streptomyces subtilisin inhibitor (SSI), suggesting that kexstatin belongs to the SSI family. Kexstatin was a strong inhibitor of Kex 2 proteinase and subtilisin but not thermolysin, trypsin, or chymotrypsin. The IC 50 value of kexstatin against 1μg of Kex 2 proteinase was 1.4μg.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.60.1388