Transient Expression of Goat Growth Hormone Gene in Poplar (Populus alba L.) Protoplasts

We developed a sensitive, accurate, and fast method to detect foreign gene expression using reverse transcriptase-mediated polymerase chain reaction (RT-PCR) in poplar tree (Populus alba L.) protoplasts. Template total RNA was purified by removing the transfected foreign gene vector completely with...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1997-09, Vol.61 (9), p.1580
Main Authors: QIAO, Jingbo, ISHIHARA, Yasuyuki, KURODA, Hiroyuki, SAKAI, Fukumi, SAKAI, Hiroshi, KOMANO, Tohru
Format: Article
Language:English
Online Access:Get full text
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Summary:We developed a sensitive, accurate, and fast method to detect foreign gene expression using reverse transcriptase-mediated polymerase chain reaction (RT-PCR) in poplar tree (Populus alba L.) protoplasts. Template total RNA was purified by removing the transfected foreign gene vector completely with DNase I treatment before the RT reaction. Expression of cDNA that encodes goat growth hormone was confirmed at the mRNA level 24h after electroporation mediated DNA transfer.
ISSN:0916-8451
1347-6947