Effect of Reaction Temperature on the Determination of Rat Serum IgE by the Avidin-Biotin Method

We established an avidin-biotin method for the sensitive determination of rat IgE and found that a non-specific signal was generated depending on the reaction temperature. When the sera of rats immunized or not with ovalbumin (OVA) were fractionated in a hydroxyapatite column and OVA-specific IgE wa...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1996, Vol.60 (11), p.1891-1892
Main Authors: Yamada, Koji, Nakao, Akemi, Takasugi, Mikako, Itoh, Yuji, Hassan, Nasra Ahmed, Sugano, Michihiro
Format: Article
Language:English
Subjects:
Allergic diseases
allergy
Animals
Avidin - chemistry
avidin-biotin method
Biological and medical sciences
Biotechnology
Biotin - chemistry
Enzyme-Linked Immunosorbent Assay
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Hydroxyapatites
IgE
Immediate hypersensitivity. Allergy. Anaphylaxis, etc
Immunobiology
Immunoglobulin E - analysis
Immunoglobulin E - isolation & purification
Immunopathology
Indicators and Reagents
Male
Medical sciences
Other localizations
Ovalbumin - immunology
Rats
Rats, Inbred BN
Techniques
Temperature
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Summary:We established an avidin-biotin method for the sensitive determination of rat IgE and found that a non-specific signal was generated depending on the reaction temperature. When the sera of rats immunized or not with ovalbumin (OVA) were fractionated in a hydroxyapatite column and OVA-specific IgE was determined by the avidin-biotin method at 4°C or 37°C, OVA-specific IgE peaks were detected at 37°C, even with nonimmunized rats, but not at 4°C.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.60.1891