Thymocyte apoptosis by T-2 toxin in vivo in mice is independent of Fas/Fas ligand system

To find whether Fas/Fas ligand (FasL) pathway is involved in T-2 toxin (T-2)-mediated thymocyte apoptosis, we used lpr/lpr (lpr) and gld/gld (gld) mice, whose Fas and FasL protein, respectively, are functionally deficient. Based on the DNA fragmentation profile in gel electrophoresis and measurement...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2000-01, Vol.64 (1), p.210-213
Main Authors: Alam, M.M. (Kagawa Univ., Miki (Japan). Faculty of Agriculture), Nagase, M, Yoshizawa, T, Sakato, N
Format: Article
Language:English
Subjects:
Animals
APOPTOSE
APOPTOSIS
Apoptosis - drug effects
CELLS
CELLULE
CELULAS
DNA Fragmentation
Electrophoresis, Polyacrylamide Gel
Fas
Fas ligand
Fas Ligand Protein
fas Receptor - metabolism
Female
Flow Cytometry
GLANDE THYMUS
GLANDULA DEL TIMO
Membrane Glycoproteins - metabolism
MICE
Mice, Inbred C3H
Mice, Inbred MRL lpr
MICOTOXINAS
MYCOTOXINE
MYCOTOXINS
RATON
SOURIS
T-2 toxin
T-2 Toxin - pharmacology
thymocyte
THYMUS GLAND
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Summary:To find whether Fas/Fas ligand (FasL) pathway is involved in T-2 toxin (T-2)-mediated thymocyte apoptosis, we used lpr/lpr (lpr) and gld/gld (gld) mice, whose Fas and FasL protein, respectively, are functionally deficient. Based on the DNA fragmentation profile in gel electrophoresis and measurement of apoptotic cell percent by flow cytometry, the levels of thymocyte apoptosis in lpr and gld mice that had received T-2 showed that both lpr and gld mice had undergone apoptosis essentially to the same magnitude as those of corresponding wild type mice (+/+). These results strongly suggest that T-2-induced thymocyte apoptosis in vivo in mice is independent of the Fas/FasL pathway
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.64.210