Cloning and sequencing of an endoglucanase gene from Scopulariopsis brevicaulis TOF-1212, and its expression in Saccharomyces cerevisiae

The egI gene, encoding a major endoglucanase (EGI) of Scopulariopsis brevicaulis TOF-1212, was cloned and sequenced. The egI gene consisted of 868 bp with one intron and encoded a protein of 229 amino acids with a calculated molecular mass of 22,392 daltons. The EGI was assigned to a family 45 of gl...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2000-06, Vol.64 (6), p.1238-1246
Main Authors: Nakatani, F. (Osaka Prefectural Univ., Sakai (Japan). Coll. of Agriculture), Kawaguchi, T, Takada, G, Sumitani, J, Moriyama, Y, Arai, M
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Ascomycota - enzymology
Ascomycota - genetics
Base Sequence
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Cellulase - biosynthesis
Cellulase - chemistry
Cellulase - genetics
Cloning, Molecular
DNA Primers - genetics
DNA, Fungal - genetics
egI gene
Endoglucanase
expression
Fundamental and applied biological sciences. Psychology
GENE EXPRESSION
Genes, Fungal
Genetics
GLYCOSIDASES
Hydrogen-Ion Concentration
Mission oriented research
MOLECULAR CLONING
Molecular Sequence Data
Molecular Weight
NUCLEOTIDE SEQUENCE
Recombinant Proteins - biosynthesis
Recombinant Proteins - chemistry
Recombinant Proteins - genetics
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
SCOPULARIOPSIS
Scopulariopsis brevicaulis
Sequence Homology, Amino Acid
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Summary:The egI gene, encoding a major endoglucanase (EGI) of Scopulariopsis brevicaulis TOF-1212, was cloned and sequenced. The egI gene consisted of 868 bp with one intron and encoded a protein of 229 amino acids with a calculated molecular mass of 22,392 daltons. The EGI was assigned to a family 45 of glycosyl hydrolases and showed high similarity with other fungal endoglucanases, especially with those of Humicola grisea and Fusarium oxysporum, on the basis of hydrophobic cluster analysis. The egI gene was expressed under the promoter of the phosphoglycerate kinase gene (PGK) in Saccharomyces cerevisiae. The transformed cells were able to secrete the enzyme efficiently in an active form.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.64.1238