Cloning and Expression of a Novel Murine Anti-human Fas Antibody

Agonistic anti-human Fas antibodies that can induce apoptosis are thought to have therapeutic effects for various diseases resulting from an abnormality of the Fas/FasL system. However, some anti-Fas antibodies show toxicity, and it is difficult to investigate their therapeutic and toxicological eff...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2000-09, Vol.64 (9), p.1903-1908
Main Authors: YOSHIDA-KATO, Hiroko, ICHIKAWA, Kimihisa, YAMAGUCHI, Junko, WATANABE, Kenji, OHSUMI, Jun, YONEHARA, Shin, SERIZAWA, Nobufusa
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Antibodies, Monoclonal - biosynthesis
Antibodies, Monoclonal - genetics
Antibodies, Monoclonal - immunology
antibody
Antigen-Antibody Complex
apoptosis
Base Sequence
Biological and medical sciences
Biotechnology
Cercopithecus aethiops
cloning
Cloning, Molecular
COS Cells
Fas
Fas antigen
fas Receptor - genetics
fas Receptor - immunology
FasL protein
Fundamental and applied biological sciences. Psychology
Health. Pharmaceutical industry
Humans
Hybridomas
Industrial applications and implications. Economical aspects
Mice
Mice, Knockout
Molecular Sequence Data
Monoclonal antibodies
Production of active biomolecules
Recombinant Proteins - biosynthesis
Recombinant Proteins - immunology
Reverse Transcriptase Polymerase Chain Reaction
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Summary:Agonistic anti-human Fas antibodies that can induce apoptosis are thought to have therapeutic effects for various diseases resulting from an abnormality of the Fas/FasL system. However, some anti-Fas antibodies show toxicity, and it is difficult to investigate their therapeutic and toxicological effect using animals because of their species specificity. We previously obtained a murine anti-human Fas mAb, HFE7A. HFE7A reacted with both human and murine Fas, and mitigated lymphadenopathy without any sign of hepatotoxicity in MRLgld/gld mice. It is suggested that humanized HFE7A would be a therapeutic treatment for various diseases resulting from an abnormality of the Fas/FasL system. Here we isolated the cDNAs that code for the heavy and light chains of HFE7A and identified the corresponding nucleotide sequences. The recombinant HFE7A was indistinguishable in binding and apoptosis-inducing activity to that from a hybridoma cell line. These data provide essential information for the humanization and clinical application of the humanized HFE7A.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.64.1903