A transgenic apple [Malus pumila] callus showing reduced polyphenol [Catechol] oxidase activity and lower browning potential

Polyphenol oxidase (PPO) is responsible for enzymatic browning of apples. Apples lacking PPO activity might be useful not only for the food industry but also for studies of the metabolism of polyphenols and the function of PPO. Transgenic apple calli were prepared by using Agrobacterium tumefaciens...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2001-02, Vol.65 (2), p.383-388
Main Authors: Murata, M. (Ochanomizu Univ., Tokyo (Japan)), Nishimura, M, Murai, N, Haruta, M, Homma, S, Itoh, Y
Format: Article
Language:English
Subjects:
Agrobacterium tumefaciens - genetics
antisense
Biological and medical sciences
Biotechnology
CATECHOL OXIDASE
Catechol Oxidase - genetics
Catechol Oxidase - metabolism
DNA, Antisense - genetics
enzymatic browning
ENZYMIC BROWNING
Fundamental and applied biological sciences. Psychology
Genes, Plant
Genetic engineering
Genetic technics
Genetic Vectors
Maillard Reaction
MALUS PUMILA
Malus×domestica (apple)
Methods. Procedures. Technologies
Plants, Genetically Modified
Plasmids - genetics
polyphenol oxidase
Rosales - enzymology
Rosales - genetics
Transgenic animals and transgenic plants
transgenic plant
TRANSGENIC PLANTS
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Summary:Polyphenol oxidase (PPO) is responsible for enzymatic browning of apples. Apples lacking PPO activity might be useful not only for the food industry but also for studies of the metabolism of polyphenols and the function of PPO. Transgenic apple calli were prepared by using Agrobacterium tumefaciens carrying the kanamycin (KM) resistant gene and antisense PPO gene. Four KM-resistant callus lines were obtained from 356 leaf explants. Among these transgenic calli, three calli grew on the medium containing KM at the same rate as non-transgenic callus on the medium without KM. One callus line had an antisense PPO gene, in which the amount and activity of PPO were reduced to half the amount and activity in non-transgenic callus. The browning potential of this line, which was estimated by adding chlorogenic acid, was also half the browning potential of non-transgenic callus.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.65.383