Transient RNAi induction against endogenous genes in Arabidopsis protoplasts using in vitro-prepared double-stranded RNA

RNA interference is a powerful technique for suppressing gene functions in many eukaryotes including plants. Here we show that introduction of double- stranded RNA into Arabidopsis protoplasts leads to marked silencing of endogenous genes, as observed previously for transgenes [Biosci. Biotechnol. B...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2005-02, Vol.69 (2), p.415-418
Main Authors: An, C.I. (Osaka Univ., Suita (Japan)), Sawada, A, Kawaguchi, Y, Fukusaki, E, Kobayashi, A
Format: Article
Language:English
Subjects:
ARABIDOPSIS
Arabidopsis - genetics
Biological and medical sciences
Cells, Cultured
double-stranded RNA
endogenous genes
EUCARYOTIC CELLS
Fundamental and applied biological sciences. Psychology
Gene Dosage
Gene Expression Regulation, Plant
Gene Silencing
GENES
GENETIC ENGINEERING
INHIBITION
PROTOPLASTS
Protoplasts - physiology
RNA
RNA Interference
RNA, Double-Stranded - physiology
Time Factors
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Summary:RNA interference is a powerful technique for suppressing gene functions in many eukaryotes including plants. Here we show that introduction of double- stranded RNA into Arabidopsis protoplasts leads to marked silencing of endogenous genes, as observed previously for transgenes [Biosci. Biotechnol. Biockem., 67, 2674-2677 (2003)]. This simple system should be useful for functional analysis of genes involved in fundamental cellular processes.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.69.415