Overexpression of the RADICAL-INDUCED CELL DEATH1 (RCD1) gene of arabidopsis [Arabidopsis thaliana] causes weak rcd1 phenotype with compromised oxidative-stress responses

rcd1 is a mutant of Arabidopsis thaliana that is more resistant to methyl viologen, but more sensitive to ozone than the wild type. rcd1-2 is caused by a single nucleotide substitution that results in a premature stop codon at Trp-332. The rcd1-2 mRNA level does not change significantly with the mut...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2006-08, Vol.70 (8), p.1827-1831
Main Authors: Fujibe, T.(Hokkaido Univ., Sapporo (Japan)), Saji, H, Watahiki, M.K, Yamamoto, K.T
Format: Article
Language:English
Subjects:
Allium cepa
Arabidopsis - drug effects
Arabidopsis - genetics
Arabidopsis Proteins - drug effects
Arabidopsis Proteins - genetics
ARABIDOPSIS THALIANA
Biological and medical sciences
Cauliflower mosaic virus
FENOTIPOS
Fundamental and applied biological sciences. Psychology
Gene Expression Profiling
GENETIC RESISTANCE
Green Fluorescent Proteins - genetics
methyl viologen (paraquat)
MUTANT
MUTANTES
MUTANTS
Mutation
Nuclear Proteins - drug effects
Nuclear Proteins - genetics
Oxidative Stress - physiology
OZONE
Ozone - pharmacology
OZONO
PARACUAT
PARAQUAT
PHENOTYPE
PHENOTYPES
RCD1
Recombinant Fusion Proteins - genetics
RESISTANCE GENETIQUE
RESISTENCIA GENETICA
Reverse Transcriptase Polymerase Chain Reaction - methods
RNA, Messenger - drug effects
RNA, Messenger - genetics
Seeds - drug effects
Seeds - genetics
subnuclear localization
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Summary:rcd1 is a mutant of Arabidopsis thaliana that is more resistant to methyl viologen, but more sensitive to ozone than the wild type. rcd1-2 is caused by a single nucleotide substitution that results in a premature stop codon at Trp-332. The rcd1-2 mRNA level does not change significantly with the mutation. Since overexpression of rcd1-1 cDNA has been shown to bring about an rcd1-like phenotype, we created and examined the overexpression lines of RCD1 by the use of the cauliflower mosaic virus 35S promoter. The transgenic lines exhibited a weak rcd1-like phenotype, although no resistance to methyl viologen was observed. Further, they fully complemented the aberrant rcd1-2 phenotype. Subcellular localization of RCD1 was examined by transiently expressing green fluorescent protein (GFP) fused with RCD1 in onion epidermal cells. GFP signals are observed as aggregated foci in the inner nuclear matrix-like region.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.50673