Anti-inflammatory effect of buckwheat [Fagopyrum esculentum] sprouts in lipopolysaccharide-activated human colon cancer cells and mice

• Published in: Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2008-12, Vol.72 (12), p.3148-3157
• Main Authors: Ishii, S.(Obihiro Univ. of Agriculture and Veterinary Medicine, Hokkaido (Japan)), Katsumura, T, Shiozuka, C, Ooyauchi, K, Kawasaki, K, Takigawa, S, Fukushima, T, Tokuji, Y, Kinoshita, M, Ohnishi, M, Kawahara, M, Ohba, K
• Format: Article
• Language: English
• Subjects: Animals; ANTI-INFLAMMATOIRE; Anti-Inflammatory Agents - administration & dosage; Anti-Inflammatory Agents - pharmacology; anti-inflammatory effect; ANTIINFLAMMATORY AGENTS; ANTINFLAMATORIOS; Biological and medical sciences; BROTES; buckwheat sprouts; Cell Line, Tumor; Cell Survival - drug effects; CITOQUINAS; Colonic Neoplasms - pathology; CYTOKINE; cytokine expression; CYTOKINES; EXPRESION GENICA; EXPRESSION DES GENES; EXTRACTOS VEGETALES; EXTRAIT D'ORIGINE VEGETALE; Fagopyrum - chemistry; FAGOPYRUM ESCULENTUM; Flavonoids - pharmacology; Fundamental and applied biological sciences. Psychology; Galactosamine - toxicity; GENE EXPRESSION; Gene Expression Regulation, Neoplastic - drug effects; Humans; Inflammation - chemically induced; Inflammation - metabolism; Interleukin-8 - genetics; lipopolysaccharide-activated cells; Lipopolysaccharides - administration & dosage; Lipopolysaccharides - toxicity; Liver - drug effects; Liver - injuries; MICE; Oligonucleotide Array Sequence Analysis; PLANT EXTRACTS; Plant Extracts - administration & dosage; Plant Extracts - pharmacology; Plant Shoots - chemistry; POUSSE; RATON; Reference Standards; SHOOTS; SOURIS; Up-Regulation - drug effects
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.80324

In conducting an in vitro screening of ethanol extracts from various natural foods using a human colon cancer cell line (CoLoTC cells), an extract of buckwheat sprouts (ExtBS) was found to express significant anti-inflammatory activity. The anti-inflammatory activity of ExtBS was confirmed by oral administration of lipopolysaccharide (LPS) to mice. Inflammatory cytokines (interleukin 6 and tumor necrosis factor alpha) were markedly up-regulated in the spleen and liver from LPS-administrated mice, and combinatory treatment with LPS and ExtBS decreased up-regulation of them in both cytokines. Both serum cytokine levels corresponded to their gene expressions in tissues, but no anti-inflammatory effect in mice was observed when ExtBS was treated intraperitoneally. ExtBS oral administration also showed protective activity as to hepatic injury induced by galactosamine/LPS treatment. Based on these data, we suggest that ExtBS contains anti-inflammatory compounds.