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Cloning, expression and purification of Bacillus cereus endochitinase in the Escherichia coli AD494(DE3)pLysS expression system
A chitinase gene from Bacillus cereus was cloned and expressed in Escherichia coli. The purified recombinant chitinase had much higher (128 fold) specificity to pNP-β-(GlcNAc) 3 than to pNP-β-(GlcNAc), suggesting endochitinase. Thirty-three amino acids in the N-terminal were recognized and cut off d...
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| Published in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2009-05, Vol.73 (5), p.1172-1174 |
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| Main Authors: | , , , |
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| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c566t-e4eb51cdbd316d22ea1dff8d2a51bd0f9f8c227b075cb9d76a3fbb4cbf953d863 |
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| container_end_page | 1174 |
| container_issue | 5 |
| container_start_page | 1172 |
| container_title | Bioscience, biotechnology, and biochemistry |
| container_volume | 73 |
| creator | Chen, W.M.(National Taiwan Ocean Univ., Keelung) Chen, G.H Chen, C.S Jiang, S.T |
| description | A chitinase gene from Bacillus cereus was cloned and expressed in Escherichia coli. The purified recombinant chitinase had much higher (128 fold) specificity to pNP-β-(GlcNAc)
3
than to pNP-β-(GlcNAc), suggesting endochitinase. Thirty-three amino acids in the N-terminal were recognized and cut off during expression, which consequently made the M
r
not correspond to that predicted. |
| doi_str_mv | 10.1271/bbb.80618 |
| format | article |
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3
than to pNP-β-(GlcNAc), suggesting endochitinase. Thirty-three amino acids in the N-terminal were recognized and cut off during expression, which consequently made the M
r
not correspond to that predicted.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.80618</identifier><identifier>PMID: 19420688</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>Amino Acid Sequence ; BACILLUS CEREUS ; Bacillus cereus - enzymology ; Base Sequence ; Biological and medical sciences ; CHITINASE ; Chitinases - biosynthesis ; Chitinases - chemistry ; Chitinases - genetics ; Chitinases - isolation & purification ; Chitinases - metabolism ; CLONACION MOLECULAR ; CLONAGE MOLECULAIRE ; Cloning, Molecular ; endochitinase ; Escherichia coli ; Escherichia coli - genetics ; EXPRESION GENICA ; EXPRESSION DES GENES ; Fundamental and applied biological sciences. Psychology ; GENE ; GENE EXPRESSION ; GENES ; MOLECULAR CLONING ; Molecular Sequence Data ; PURIFICACION ; PURIFICATION ; QUITINASA ; recognition peptide</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2009-05, Vol.73 (5), p.1172-1174</ispartof><rights>2009 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 2009</rights><rights>2009 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c566t-e4eb51cdbd316d22ea1dff8d2a51bd0f9f8c227b075cb9d76a3fbb4cbf953d863</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21661068$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19420688$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, W.M.(National Taiwan Ocean Univ., Keelung)</creatorcontrib><creatorcontrib>Chen, G.H</creatorcontrib><creatorcontrib>Chen, C.S</creatorcontrib><creatorcontrib>Jiang, S.T</creatorcontrib><title>Cloning, expression and purification of Bacillus cereus endochitinase in the Escherichia coli AD494(DE3)pLysS expression system</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>A chitinase gene from Bacillus cereus was cloned and expressed in Escherichia coli. The purified recombinant chitinase had much higher (128 fold) specificity to pNP-β-(GlcNAc)
3
than to pNP-β-(GlcNAc), suggesting endochitinase. Thirty-three amino acids in the N-terminal were recognized and cut off during expression, which consequently made the M
r
not correspond to that predicted.</description><subject>Amino Acid Sequence</subject><subject>BACILLUS CEREUS</subject><subject>Bacillus cereus - enzymology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>CHITINASE</subject><subject>Chitinases - biosynthesis</subject><subject>Chitinases - chemistry</subject><subject>Chitinases - genetics</subject><subject>Chitinases - isolation & purification</subject><subject>Chitinases - metabolism</subject><subject>CLONACION MOLECULAR</subject><subject>CLONAGE MOLECULAIRE</subject><subject>Cloning, Molecular</subject><subject>endochitinase</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE</subject><subject>GENE EXPRESSION</subject><subject>GENES</subject><subject>MOLECULAR CLONING</subject><subject>Molecular Sequence Data</subject><subject>PURIFICACION</subject><subject>PURIFICATION</subject><subject>QUITINASA</subject><subject>recognition peptide</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNqF0kuLFDEQAOBGFHdcPfgDlIAoLthrXp10H9fZ8cWAgnoOee5kySRt0s3unPzrZp3xgQieCoqvqkhVmuYhgqcIc_RSKXXaQ4b6W80CEcpbNlB-u1nAAbG2px06au6VcglhTXTobnOEBooh6_tF820ZUvTx4gWw12O2pfgUgYwGjHP2zms53SSSA6-k9iHMBWibbQ02mqQ3fvJRFgt8BNPGglXRG5t9zUugU_Dg7JwO9Pn5ipyM61359OeQsiuT3d5v7jgZin1wiMfNl9erz8u37frDm3fLs3WrO8am1lKrOqSNMgQxg7GVyDjXGyw7pAx0g-s1xlxB3mk1GM4kcUpRrdzQEdMzctw82_cdc_o62zKJrS_ahiCjTXMRjGPeQf5_WPdGISK8wid_wcs051gfIRClQ09xT0hVJ3ulcyolWyfG7Lcy7wSC4uZ4oh5P_DhetY8PHWe1tea3PFyrgqcHIIuWwWUZtS-_HEaMoQqro3vno0t5K69SDkZMchdS_llE_jX_0b7MySTkRa7q_UdcPw2EBNfVfAeADL1f</recordid><startdate>20090501</startdate><enddate>20090501</enddate><creator>Chen, W.M.(National Taiwan Ocean Univ., Keelung)</creator><creator>Chen, G.H</creator><creator>Chen, C.S</creator><creator>Jiang, S.T</creator><general>Japan Society for Bioscience, Biotechnology, and Agrochemistry</general><general>Japan Society for Bioscience Biotechnology and Agrochemistry</general><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20090501</creationdate><title>Cloning, expression and purification of Bacillus cereus endochitinase in the Escherichia coli AD494(DE3)pLysS expression system</title><author>Chen, W.M.(National Taiwan Ocean Univ., Keelung) ; Chen, G.H ; Chen, C.S ; Jiang, S.T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c566t-e4eb51cdbd316d22ea1dff8d2a51bd0f9f8c227b075cb9d76a3fbb4cbf953d863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Amino Acid Sequence</topic><topic>BACILLUS CEREUS</topic><topic>Bacillus cereus - enzymology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>CHITINASE</topic><topic>Chitinases - biosynthesis</topic><topic>Chitinases - chemistry</topic><topic>Chitinases - genetics</topic><topic>Chitinases - isolation & purification</topic><topic>Chitinases - metabolism</topic><topic>CLONACION MOLECULAR</topic><topic>CLONAGE MOLECULAIRE</topic><topic>Cloning, Molecular</topic><topic>endochitinase</topic><topic>Escherichia coli</topic><topic>Escherichia coli - genetics</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE</topic><topic>GENE EXPRESSION</topic><topic>GENES</topic><topic>MOLECULAR CLONING</topic><topic>Molecular Sequence Data</topic><topic>PURIFICACION</topic><topic>PURIFICATION</topic><topic>QUITINASA</topic><topic>recognition peptide</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, W.M.(National Taiwan Ocean Univ., Keelung)</creatorcontrib><creatorcontrib>Chen, G.H</creatorcontrib><creatorcontrib>Chen, C.S</creatorcontrib><creatorcontrib>Jiang, S.T</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, W.M.(National Taiwan Ocean Univ., Keelung)</au><au>Chen, G.H</au><au>Chen, C.S</au><au>Jiang, S.T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning, expression and purification of Bacillus cereus endochitinase in the Escherichia coli AD494(DE3)pLysS expression system</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>2009-05-01</date><risdate>2009</risdate><volume>73</volume><issue>5</issue><spage>1172</spage><epage>1174</epage><pages>1172-1174</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>A chitinase gene from Bacillus cereus was cloned and expressed in Escherichia coli. The purified recombinant chitinase had much higher (128 fold) specificity to pNP-β-(GlcNAc)
3
than to pNP-β-(GlcNAc), suggesting endochitinase. Thirty-three amino acids in the N-terminal were recognized and cut off during expression, which consequently made the M
r
not correspond to that predicted.</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>19420688</pmid><doi>10.1271/bbb.80618</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0916-8451 |
| ispartof | Bioscience, biotechnology, and biochemistry, 2009-05, Vol.73 (5), p.1172-1174 |
| issn | 0916-8451 1347-6947 |
| language | eng |
| recordid | cdi_proquest_journals_1449842833 |
| source | Oxford Journals Online; EZB Electronic Journals Library |
| subjects | Amino Acid Sequence BACILLUS CEREUS Bacillus cereus - enzymology Base Sequence Biological and medical sciences CHITINASE Chitinases - biosynthesis Chitinases - chemistry Chitinases - genetics Chitinases - isolation & purification Chitinases - metabolism CLONACION MOLECULAR CLONAGE MOLECULAIRE Cloning, Molecular endochitinase Escherichia coli Escherichia coli - genetics EXPRESION GENICA EXPRESSION DES GENES Fundamental and applied biological sciences. Psychology GENE GENE EXPRESSION GENES MOLECULAR CLONING Molecular Sequence Data PURIFICACION PURIFICATION QUITINASA recognition peptide |
| title | Cloning, expression and purification of Bacillus cereus endochitinase in the Escherichia coli AD494(DE3)pLysS expression system |
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