Turnover Ability of a Designed RNA Acting as a Template for Chemical Peptide Ligation

A designed self-folding RNA possessing two peptide-recognition motifs served as a template for the chemical ligation of two RNA-binding peptides under stoichiometric conditions. In this study, we investigated the turnover ability of this template RNA in facilitation of peptide ligation and found tha...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2011, Vol.75 (10), p.2021-2024
Main Authors: YAMASHITA, Kohei, KASHIWAGI, Norimasa, FURUTA, Hiroyuki, IKAWA, Yoshiya
Format: Article
Language:English
Subjects:
Base Sequence
Biochemistry
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Models, Molecular
N-peptide
native chemical ligation
P4-P6 RNA
Peptides
Peptides - chemistry
Peptides - metabolism
Rev-peptide
Ribonucleic acids
RNA
RNA - chemistry
RNA - genetics
RNA - metabolism
RNA Folding
RNA Stability
RNA-Binding Proteins - chemistry
RNA-Binding Proteins - metabolism
Three dimensional
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Summary:A designed self-folding RNA possessing two peptide-recognition motifs served as a template for the chemical ligation of two RNA-binding peptides under stoichiometric conditions. In this study, we investigated the turnover ability of this template RNA in facilitation of peptide ligation and found that the RNA exhibited modest turnover ability under conditions in which its 3D structure was marginally stable.
ISSN:0916-8451
1347-6947
1347-6947
DOI:10.1271/bbb.110255