Determination of TiO^sub 2^, ZrO^sub 2^, and Al^sub 2^O^sub 3^ Nanoparticles on Genotoxic Responses in Human Peripheral Blood Lymphocytes and Cultured Embyronic Kidney Cells

In this study a genotoxic evaluation of titanium dioxide (..., 2.3 nm), zirconium oxide (..., 6 nm), aluminum oxide (..., 16.7 nm) nanoparticles (NP) and their ionic forms was conducted using human peripheral blood lymphocytes and cultured human embryonic kidney (HEK293) cells by means of a modified...

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Bibliographic Details
Published in:Journal of toxicology and environmental health. Part A 2013-08, Vol.76 (16), p.990
Main Authors: Demir, Esref, Burgucu, Durmus, Turna, Fatma, Aksakal, Sezgin, Kaya, Bülent
Format: Article
Language:English
Subjects:
Cell culture
Kidneys
Lymphocytes
Nanoparticles
Titanium
Toxicity
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Summary:In this study a genotoxic evaluation of titanium dioxide (..., 2.3 nm), zirconium oxide (..., 6 nm), aluminum oxide (..., 16.7 nm) nanoparticles (NP) and their ionic forms was conducted using human peripheral blood lymphocytes and cultured human embryonic kidney (HEK293) cells by means of a modified alkaline comet assay with/without the formamidopyrimidine-DNA glycosylase (Fpg) and endonuclease III (Endo III) enzymes. Modifications to the comet assay by using lesion-specific endonucleases, such as Endo III and Fpg, detect DNA bases with oxidative damage. Both human peripheral blood lymphocytes and cultured embryonic kidney cells were incubated with ..., ..., or ... NP at concentrations of 1, 10, or 100 ...g/ml. Our results showed no significant induction in DNA damage by the comet assay with/without the Endo III and Fpg enzymes at all concentrations of ... and ... In the case of ... NP only the highest concentration of 100 ...g/ml significantly induced a genotoxic response. Data thus indicate that both ... and ... NP were not genotoxic in our system and in the case of ... the lowest-observed-adverse-effect level (LOAEL) for genotoxicity was 100 ...g/ml. Evidence indicates that these metallic NP are considered safe in light of the fact that no genotoxicity was noted with ... and ... and that the highest ... concentration is not environmentally relevant. (ProQuest: ... denotes formulae/symbols omitted.)
ISSN:1528-7394
2381-3504