RESVEGA, by the presence of Resveratrol, inhibits retinal endothelial tube formation

• Published in: Acta ophthalmologica (Oxford, England) England), 2013-08, Vol.91 (s252), p.0-0
• Main Authors: OLMIERE, C, CLERC, A, LECONTE, L
• Format: Article
• Language: English
• Subjects: Ophthalmology
• ISSN: 1755-375X; 1755-3768
• DOI: 10.1111/j.1755-3768.2013.F015.x

Purpose Resveratrol (RES) is a polyphenol with various properties. RESVEGA, a food supplement developed by Thea, contains 2% of RES. The aim of this study was to examine the activity of RES and RESVEGA on endothelial cells. Methods Compounds activity was tested using an in vitro angiogenesis assay. Endothelial cells obtained from human umbilical vein (HUVEC) and monkey retinal endothelial cells (RF/6A) were used for this assay. 96‐well cell cultures plates were coated with Matrigel. Cells (15000 per well) were incubated in the presence of RES, RESVEGA or vehicle. Tube formation was recorded every 15 min during 5 hours using Time Lapse Video Microscopy. At each time point, 3 fields per well were photographed and images were analysed using Metamorph software. Each experiment was repeated three times, with triplicate samples. Results RES and RESVEGA products activities were compared at the following concentrations: 25, 50 and 100 µM. Both RES, tested on HUVEC and RF/6A, and RESVEGA, tested on RF/6A, reduced the ability of endothelial cells to form vascular networks on Matrigel. This inhibitory effect was dose dependent in the range of 25‐100 µM. Conclusion This is the first report of the anti‐angiogenic effect of RES on RF/6A retinal endothelial cells. RESVEGA supplement presents the same anti‐angiogenic activity than resveratrol alone. Commercial interest