Introduction of a Highly Photodurable and Common-laser Excitable Fluorescent Amino Acid into a Peptide as a FRET Acceptor for Protease Cleavage Detection

Synthesis and photochemical properties of a benzoacridone-containing fluorescent amino acid (badAla) which is photodurable and excitable by widely applicable 488 nm lasers was described. The amino acid carries a relatively small fluorophore that shows absorption around 450–500 nm, emission above 500...

Full description

Saved in:
Bibliographic Details
Published in:Chemistry letters 2010-08, Vol.39 (8), p.818-819
Main Authors: Taki, Masumi, Yamazaki, Yoshito, Suzuki, Yuto, Sisido, Masahiko
Format: Article
Language:English
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Synthesis and photochemical properties of a benzoacridone-containing fluorescent amino acid (badAla) which is photodurable and excitable by widely applicable 488 nm lasers was described. The amino acid carries a relatively small fluorophore that shows absorption around 450–500 nm, emission above 500 nm with a high fluorescence quantum yield (0.65), and relatively long fluorescence lifetime (17 ns). BadAla can be used in solid-phase peptide synthesis without any precaution. A peptide-containing badAla was used to measure caspase activity via fluorescence resonance energy transfer (FRET).
ISSN:0366-7022
1348-0715
DOI:10.1246/cl.2010.818