Production of p-Aminosalicylic Acid through Enzymatic Kolbe–Schmitt Reaction Catalyzed by Reversible Salicylic Acid Decarboxylase

A reversible salicylic acid decarboxylase (Sdc), found in the yeast Trichosporon moniliiforme WU-0401, is applicable for the production of p-aminosalicylic acid (PAS) from m-aminophenol (m-AP). For the high-yield production of PAS, used as an antituberculous agent, we developed F195Y, a genetically...

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Bibliographic Details
Published in:Chemistry letters 2011-02, Vol.40 (2), p.206-208
Main Authors: Kirimura, Kohtaro, Yanaso, Satomi, Kosaka, Sachiyo, Koyama, Keiko, Hattori, Takasumi, Ishii, Yoshitaka
Format: Article
Language:English
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Summary:A reversible salicylic acid decarboxylase (Sdc), found in the yeast Trichosporon moniliiforme WU-0401, is applicable for the production of p-aminosalicylic acid (PAS) from m-aminophenol (m-AP). For the high-yield production of PAS, used as an antituberculous agent, we developed F195Y, a genetically engineered Sdc mutant. We succeeded in selectively producing PAS from m-AP through an enzymatic Kolbe–Schmitt reaction in aqueous solution by using recombinant Escherichia coli cells expressing the gene encoding F195Y. We found that 70 mM PAS was produced at 30 °C in 15 h with a conversion yield of 70% (mol/mol).
ISSN:0366-7022
1348-0715
DOI:10.1246/cl.2011.206