Analytical Method Development and Validation for Simultaneous Determination of Ambroxol and Olopatadine Hydrochloride in Synthetic Mixture

An Absorption Correction UV spectrophotometric and a reversed Phase high-performance liquid chromatographic method for the determination of Ambroxol and Olopatadine Hydrochloride in Synthetic mixture were developed. The Absorption Correction UV spectrophotometric method was found to be accurate with...

Full description

Saved in:
Bibliographic Details
Published in:Asian Journal of Research in Chemistry 2013-04, Vol.6 (4), p.IX
Main Authors: Limbachiya, Ujjaval N, Patel, Nikita N, Nikesh, Rana, Rajesh, K S, Patel, Shreya, Shinde, Gajanan
Format: Article
Language:English
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An Absorption Correction UV spectrophotometric and a reversed Phase high-performance liquid chromatographic method for the determination of Ambroxol and Olopatadine Hydrochloride in Synthetic mixture were developed. The Absorption Correction UV spectrophotometric method was found to be accurate with 99.75±0.04 recovery for ambroxol and recovery for olopatadine 99.89±0.02 respectively, and method is also precise with a coefficient of variation for olopatadine and ambroxol was 0.996 and 0.885 respectively. For reverse phase HPLC, A reversed-phase C^sub 18^ column with methanol: ammonium acetate buffer (80:20, v/v, pH 5.5) mobile phase was used, and the detector wavelength was set at 244 nm. Linearity range for ambroxol and Olopatadine Hcl was found to be 78-150 μg/ml and 13-25 μg/ml respectively.
ISSN:0974-4169
0974-4150