New cellular RNA elimination method for cell-based SELEX of modified RNA aptamers

Davydova et al developed a new efficient method for separation of 2' modified RNA aptamers from a mixture with total cellular RNA during cell based SELEX using artificial ribonucleases. The possibilities of the method are shown for 2' F modified RNA aptamers that are able to bind human IGF...

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Bibliographic Details
Published in:Molecular biology (New York) 2013-11, Vol.47 (6), p.904-906
Main Authors: Davydova, A. S., Vorobjeva, M. A., Zenkova, M. A., Silnikov, V. N., François, J. -C., Venyaminova, A. G.
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Cellular biology
Enzymes
Human Genetics
Life Sciences
Molecular biology
Ribonucleic acid
RNA
Short Communications
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Summary:Davydova et al developed a new efficient method for separation of 2' modified RNA aptamers from a mixture with total cellular RNA during cell based SELEX using artificial ribonucleases. The possibilities of the method are shown for 2' F modified RNA aptamers that are able to bind human IGF I receptor on the cell surface.
ISSN:0026-8933
1608-3245
DOI:10.1134/S0026893313060034