A SIGNIFICANT RELATIONSHIP BETWEEN GLUTATHIONE-PEROXIDASH (GSH-PC) LOCALIZATION AND BIOLOGICAL ACTION OF TESTOSTERONE IN RAT VENTRAL PROSTATE

• Published in: Journal of toxicological sciences 1999/08/17, Vol.24(3), pp.209-216
• Main Authors: MURAKOSHI, Masanori, IKEDA, Rie, TAGAWA, Masashi
• Format: Article
• Language: English
• Subjects: Rat
• ISSN: 0388-1350; 1880-3989
• DOI: 10.2131/jts.24.3_209

In order to confirm the relationship between glutathione-peroxidase (GSH-PO) localization and biological testosterone action in the rat ventral prostate, immunocytochemical localization of GSH-PO in glandular epithelial cells of the rat ventral prostate was investigated. In the untreated group, GSH-PO was predominantly demonstrated in glandular epithelial cells of the ventral prostate. Intracellular localization of GSH-PO in the glandular epithelial cells was mainly observed in cytoplasmic matrix near the rough endoplasmic reticulum and was occasionally noted as a small granular structure (GSH-PO-positive granule) at the supranuclear region. In a castrated animal, the intensity of GSH-PO staining in the glandular epithelial cells was remarkably decreased. By testosterone administration to the castrated animal, GSH-PO was clearly detected in the glandular epithelial cells. Intracellular localization of GSH-PO was mainly observed in cytoplasmic matrix and the number of GSH-PO-positive granules increased remarkably. These findings suggest that immunostainable GSH-PO in the glandular epithelial cells of the rat ventral prostate is testosterone-dependent, and that its staining patter is a useful marker for biological testosterone action.