Analgesic effects of mad honey (grayanotoxin) in mice models of acute pain and painful diabetic neuropathy

• Published in: Human & experimental toxicology 2014-02, Vol.33 (2), p.130-135
• Main Authors: Gunduz, A, Eraydin, I, Turkmen, S, Kalkan, O F, Turedi, S, Eryigit, U, Ayar, A
• Format: Article
• Language: English
• Subjects: Alternative medicine; Analgesics; Analgesics - pharmacology; Animals; Antibacterial agents; Antibiotics. Antiinfectious agents. Antiparasitic agents; Biological and medical sciences; Cranial nerves. Spinal roots. Peripheral nerves. Autonomic nervous system. Gustation. Olfaction; Diabetes Mellitus, Experimental - complications; Diabetes. Impaired glucose tolerance; Diabetic Neuropathies - chemically induced; Diabetic neuropathy; Dose-Response Relationship, Drug; Drug Administration Schedule; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; Etiopathogenesis. Screening. Investigations. Target tissue resistance; Honey; Hot Temperature - adverse effects; Male; Medical sciences; Mice; Mice, Inbred BALB C; Nervous system (semeiology, syndromes); Neurology; Pain - chemically induced; Pain management; Pharmacology. Drug treatments; Random Allocation; Studies; Toxicology; Toxins, Biological - pharmacology
• ISSN: 0960-3271; 1477-0903
• DOI: 10.1177/0960327113482693

Objectives: The aim of this experimental study was to investigate the effects of mad honey (grayanotoxin, GTX), used in complementary medicine for a variety of purposes besides being food, on pain thresholds in normal mice as model for acute pain and diabetic mouse as model for neuropathic pain. Methods: Hind paw withdrawal pain threshold to thermal stimulus was measured with a plantar analgesia meter in a mice model using healthy intact animals for acute pain and streptozotocin-induced diabetic animals for chronic neuropathic pain. Time and dose-dependent effects of intraperitoneally (i.p.) administered GTX were investigated in both acute and neuropathic pain. Results: In the acute pain model, administration of GTX caused a dose- and time-dependent marked increase in the pain latency values. In diabetic mice, which had markedly increased threshold to pain, GTX (0.1 mg/kg, i.p.) restored the mean pain latencies by decreasing from the pre-GTX treatment values of 3.2 ± 0.6 to 3.0 ± 0.9s at 10 min, 3.2 ± 0.6s at 20 min, 3.4 ± 0.6s at 30 min, 2.6 ± 0.5s at 60 min and 2.4 ± 0.6s (p