Inactivation mechanisms of pathogenic bacteria in several matrixes during the composting process in a composting toilet

• Published in: Environmental technology 2014-03, Vol.35 (6), p.674-680
• Main Authors: Sossou, S.K, Hijikata, N, Sou, M, Tezuka, R, Maiga, A.H, Funamizu, N
• Format: Article
• Language: English
• Subjects: agar; Agar - chemistry; alkaline pH; Animals; Applied sciences; Bacteria; Bacteria - growth & development; Bacteriology; Biodegradation, Environmental; charcoal; Charcoal - chemistry; Composting; composting matrixes; composting toilet; Drinking water and swimming-pool water. Desalination; environmental technology; enzymes; Escherichia coli; Escherichia coli - growth & development; Exact sciences and technology; Feces; General treatment and storage processes; Glycine max - metabolism; Hydrogen-Ion Concentration; inactivation mechanism; Oryza; Oryza sativa; pathogenic bacteria; Pollution; rice hulls; sawdust; Sewage; Soil; Swine; Trypsin - chemistry; Wastes; Water treatment and pollution; Wood
• ISSN: 1479-487X; 0959-3330; 1479-487X
• DOI: 10.1080/09593330.2013.841268

This study aimed to compare the inactivation rate and the mechanisms of pathogenic bacteria in three matrixes (sawdust, rice husk and charcoal) during the composting process. The inactivation rate was evaluated with Escherichia coli strain and the damaged parts and/or functions were evaluated with three different media. Normalized inactivation rate constant in three media and from three matrixes had no significant difference in each process (pure, 1 month and 2 months). The value in rice husk was relatively increased during 2 months but there was no significant difference. The inactivation rate constants of Tryptic Soy Agar (TSA) and Compact Dry E. coli /Coliform in pure sawdust and rice husk were relatively lower than that of Desoxycholate Agar, but increased in 2 months. This indicated that damaging part was changed from outer membrane to enzymes and metabolisms during the 2-month composting process. In the case of charcoal, only the TSA value in a pure matrix was relatively lower than that of others, but it increased in 2 months. This indicated that damaging part was changed from outer membrane and enzyme to metabolisms during the composting process. Composting matrix and composting process did not significantly affect inactivation rate of pathogenic bacteria during the process but affected the damaging part of the bacteria.