Sauchinone, a Lignan from Saururus chinensis, Protects Human Skin Keratinocytes against Ultraviolet B-Induced Photoaging by Regulating the Oxidative Defense System

• Published in: Biological & pharmaceutical bulletin 2013/07/01, Vol.36(7), pp.1134-1139
• Main Authors: Park, Gunhyuk, Kim, Hyo Geun, Sim, Yeomoon, Sung, Sang Hyun, Oh, Myung Sook
• Format: Article
• Language: English
• Subjects: antioxidative; antiphotoaging; Benzopyrans - isolation & purification; Benzopyrans - pharmacology; Cell Line; Cell Survival - drug effects; Cell Survival - radiation effects; Collagen Type I - metabolism; Dioxoles - isolation & purification; Dioxoles - pharmacology; Dose-Response Relationship, Drug; Humans; Keratinocytes - drug effects; Keratinocytes - enzymology; Keratinocytes - radiation effects; Matrix Metalloproteinase 1 - biosynthesis; matrix metalloproteinase-1; Molecular Structure; Radiation-Protective Agents - isolation & purification; Radiation-Protective Agents - pharmacology; sauchinone; Saururaceae - chemistry; Skin Aging - drug effects; type 1 collagen; ultraviolet B; Ultraviolet Rays - adverse effects
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.b13-00101

Ultraviolet (UV) radiation from sunlight induces matrix metalloproteinase (MMP) expression, which are responsible for collagenous extracellular matrix proteins breakdown in skin, causing photoaging. Sauchinone is reported to have various bioactivity such as antioxidative, hepatoprotective, and anti-inflammatory effects. In the present study, we investigated the protective effect of sauchinone against UVB (50 mJ/cm2)-induced photoaging in HaCaT human epidermal keratinocytes. Sauchinone, at 5–40 µ M , significantly protected keratinocytes against UVB-induced damage as assessed by cell viability and toxicity assay. Additionally, sauchinone, at 20–40 µ M , prevented the upregulation of MMP-1 proteins and reduction of type 1 collagen induced by UVB. Other assays revealed that, in keratinocytes, sauchinone decreased reactive oxygen species (ROS) production and increased glutathione levels and heme oxygenase-1. Sauchinone also inhibited UVB-induced phosphorylation of mitogen-activated protein kinase (MAPK) signaling pathways. These results demonstrated that sauchinone protects skin keratinocytes through inhibition of extracellular signal-regulated kinase, c-Jun N-terminal kinase, and p38 MAPK signaling via upregulation of oxidative defense enzymes.