Improvement of Reticuline Productivity from Dopamine by Using Engineered Escherichia coli

Benzylisoquinoline alkaloids (BIAs) are pharmaceutically important compounds. We have previously devised a reticuline (BIA) production method from dopamine by using Escherichia coli; however, its productivity was relatively low (33 µM, 11 mg/L). We report here, by fine-tuning the method, higher reti...

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Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2013, Vol.77 (10), p.2166-2168
Main Authors: KIM, Ju-Sung, NAKAGAWA, Akira, YAMAZAKI, Yuya, MATSUMURA, Eitaro, KOYANAGI, Takashi, MINAMI, Hiromichi, KATAYAMA, Takane, SATO, Fumihiko, KUMAGAI, Hidehiko
Format: Article
Language:English
Subjects:
(S)-norcoclaurine synthase
benzylisoquinoline alkaloid
Benzylisoquinolines - metabolism
Dopamine - metabolism
Escherichia coli
Escherichia coli - genetics
Escherichia coli - metabolism
Fermentation
Metabolic Engineering - methods
microbial production
monoamine oxidase
reticuline
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Summary:Benzylisoquinoline alkaloids (BIAs) are pharmaceutically important compounds. We have previously devised a reticuline (BIA) production method from dopamine by using Escherichia coli; however, its productivity was relatively low (33 µM, 11 mg/L). We report here, by fine-tuning the method, higher reticuline productivity of 165 µM (54 mg/L), increasing the conversion efficiency by 8-fold. These results are important for developing an efficient route to fermentative reticuline production.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.130552